Latrophilin 1 antibody (Extracellular, N-Term)

Catalog No. ABIN7884733

The Rabbit Polyclonal anti-Latrophilin 1 antibody is suitable to detect Latrophilin 1 in samples from Rat, Human and Mouse. It has been validated for WB, IF, IHC, FACS, IC and LCI.

Quick Overview for Latrophilin 1 antibody (Extracellular, N-Term) (ABIN7884733)

Target: Latrophilin 1 (LPHN1)

Reactivity: Rat, Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Latrophilin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunochromatography (IC), Live Cell Imaging (LCI)

Grade: Carrier-free

Product Details anti-Latrophilin 1 Antibody

Binding Specificity: AA 480-494, Extracellular, N-Term

Purpose: A Rabbit  Polyclonal Antibody to Latrophilin-1 Receptor

Predicted Reactivity: Mouse,human - identical

Purification: Affinity purified on immobilized antigen.

Immunogen: CEPREVRRVQWPATQ(G), corresponding to amino acid residues 480-494 of rat Latrophilin-1

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 2.5 μg per reaction

ICC: 1:50

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235844)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Latrophilin 1

Target: Latrophilin 1 (LPHN1)

Alternative Name: Calcium-independent alpha-latrotoxin receptor 1

Background:

Synonyms: Calcium-independent alpha-latrotoxin receptor 1, CIRL-1, Adhesion G protein-coupled receptor L1, ADGRL1, Lectomedin-2, LEC2

Description: Latrophilins (Latrophilin1-3) are members of the adhesion G-protein coupled receptor subfamily. Like all GPCRs, Latrophilins have seven transmembrane domains and are distinguished by a large extracellular N-terminal tail and a large intracellular C-terminal tail1. The N-terminus has several cell adhesion domains and undergoes proteolysis after synthesis, while the C-terminal has various consensus post-translational sites like phosphorylation and palmitoylation2. In addition, Latrophilins undergo alternative splicing3.Latrophilin-1 was discovered by its ability to bind α-Latrotoxin (α-LTX), a toxin isolated from the black widow spider venom4. α-LTX induces exocytosis by creating a Ca2+ influx in the presynaptic membrane. α-LTX can also stimulate small vesicle exocytosis in a Ca2+ independent manner. Three receptors have been found to bind α-LTX. Of the three, Latrophilins are responsible for the Ca2+-independent effects of α-LTX2. The binding of α-LTX to Latrophilin-1 increases exocytosis of neurotransmitters5,6.In an attempt to find the natural ligand of Latrophilin-1, Lasso, a splice variant of Teneneurin-2 was discovered to be an endogenous binding partner of the adhesion-GPCR7. Teneneurins are large glycoproteins with a single transmembrane domain8,9. Like Latrophilin-1, Teneneurins are mostly expressed in the brain where they modulate neurite outgrowth, axon guidance and synaptogenesis7.Regarding the localization of Latrophilins, Latrophilin-1 is expressed predominantly in the brain, Latrophilin-2 is highly expressed in the liver and lung, while Latrophilin-3 is almost exclusively detected in the brain1,10.

Gene ID: 65096

UniProt: O88917

Pathways: Synaptic Membrane, Synaptic Vesicle Exocytosis