Anoctamin 6 antibody (2nd Extracellular Loop)

Catalog No. ABIN7884761

The Rabbit Polyclonal anti-Anoctamin 6 antibody (ABIN7884761) specifically detects Anoctamin 6 in WB, FACS and LCI. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Anoctamin 6 antibody (2nd Extracellular Loop) (ABIN7884761)

Target: Anoctamin 6 (ANO6)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Anoctamin 6 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Grade: Carrier-free

Product Details anti-Anoctamin 6 Antibody

Binding Specificity: 2nd Extracellular Loop, AA 487-500

Purpose: A Rabbit Polyclonal Antibody to Anoctamin-6 Channel

Predicted Reactivity: Mouse,rat - 12,14 amino acid residues identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)KLPKNINGTDPIQK, corresponding to amino acid residues 487-500 of human ANO6

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7234747)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Anoctamin 6

Target: Anoctamin 6 (ANO6)

Alternative Name: ANO6

Background:

Synonyms: ANO6, Transmembrane protein 16F, TMEM16F, Small-conductance calcium-activated nonselective cation channel, SCAN channel

Description: Anoctamin-6 (ANO6, TMEM16F) is a member of the Anoctamin chloride channel family. This family includes 10 proteins: ANO1-ANO10 that are encoded by the conserved TMEM16A-K genes. Anoctamin-6 acts as calcium (Ca2+)-activated chloride (Cl-) channel. It generates an outwardly rectifying Cl- current when activated by a large increase in local intracellular Ca2+ concentrations1. ANO6 structure includes eight transmembrane domains and cytosolic amino- and carboxyl termini. There is a high sequence identity in the putative transmembrane domains and more divergent in other regions2,3.ANO6 has also been shown to operate as a Ca2+-activated phospholipid scramblase resulting in movement of phosphatidylserine from the inner to the outer leaflet of the plasma membrane bilayer. ANO6 is also involved in bone mineralization, cell volume regulation, cell proliferation and apoptosis1,2. ANO6 is expressed in cyst-forming epithelial cells together with ANO1.A mutation in the ANO6 gene is linked to Scott Syndrome, a rare inherited bleeding disorder, characterized by altered Ca2+-dependent platelet signaling with defective phosphatidylserine exposure and microparticle formation. Deletion of ANO6 in mice shows reduced skeleton size, skeletal deformities, and mineralization defects4,5.

Gene ID: 196527

UniProt: Q4KMQ2

Pathways: SARS-CoV-2 Protein Interactome