CALCRL antibody (Extracellular, N-Term)

Catalog No. ABIN7884807

This Rabbit Polyclonal antibody specifically detects CALCRL in WB, IHC, FACS and LCI. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CALCRL antibody (Extracellular, N-Term) (ABIN7884807)

Target: CALCRL (Calcitonin Receptor-Like (CALCRL))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CALCRL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Grade: Carrier-free

Product Details anti-CALCRL Antibody

Binding Specificity: AA 127-140, Extracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to Calcitonin Receptor-Like Receptor

Predicted Reactivity: Mouse,rat - 12,14 amino acid residues identical

Purification: Affinity purified on immobilized antigen.

Immunogen: CNVNTHEKVKTALN, corresponding to amino acid residues 127 - 140 of human Calcitonin Receptor-Like Receptor 

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7234904)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for CALCRL

Target: CALCRL (Calcitonin Receptor-Like (CALCRL))

Alternative Name: Calcitonin Receptor-Like Receptor

Background:

Synonyms: Calcitonin Receptor-Like Receptor, Calcitonin Gene-Related Peptide Type 1 Receptor, CRL, CGRPR

Description: Calcitonin Receptor-Like Receptor 1 (CRLR or CGRPR) is a G protein-coupled receptor that binds the peptide hormone calcitonin and is involved in maintenance of calcium homeostasis, particularly with respect to bone formation and metabolism1.When engaged with its substrate, calcitonin gene related peptide (CGRP), CGRPR activates cAMP-dependent pkA and PI3 kinase, which eventually leads to complex inhibitory as well as facilitator actions on Neuronal Nicotinic Acetylcholine Receptor1.CGRP receptor is a heterodimer composed of two proteins: a 7-transmembrane domain type 2 G-protein coupled receptor called calcitonin-receptor-like receptor (CRLR), and receptor-activity-modifying protein 1 (RAMP1), a single-membrane-pass protein. RAMP1 serves as a transporter for CRLR to the cell surface and its absence leads to failure of binding with CRLR2.CGRP and CGRPR are ubiquitously expressed and their interaction is related to various diseases and health states such as migraines3 and Alzheimer's disease1.

Gene ID: 10203

UniProt: Q16602

Pathways: cAMP Metabolic Process