CABP1 antibody (C-Term, Intracellular)

Catalog No. ABIN7884808

The Rabbit Polyclonal anti-CABP1 antibody is suitable to detect CABP1 in samples from Human, Mouse and Rat. It has been validated for WB.

Quick Overview for CABP1 antibody (C-Term, Intracellular) (ABIN7884808)

Target: CABP1 (Calcium Binding Protein 1 (CABP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CABP1 antibody is un-conjugated

Application: Western Blotting (WB)

Grade: Carrier-free

Product Details anti-CABP1 Antibody

Binding Specificity: AA 256-270, C-Term, Intracellular

Purpose: A Rabbit Polyclonal Antibody to Calcium-Binding Protein 1

Predicted Reactivity: Mouse,human - identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)REAMRKLLGHQVGHR, corresponding to amino acid residues 256-270 of rat CaBP1

Isotype: IgG

Application Details

Application Notes:

WB: 1:600

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7234907)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for CABP1

Target: CABP1 (Calcium Binding Protein 1 (CABP1))

Alternative Name: Calcium-binding protein 1

Background:

Synonyms: Calcium-binding protein 1, Caldendrin, Calbrain

Description: Neuronal Ca2+-binding proteins (CaBP1-5) are a subclass of the calmodulin (CaM) superfamily that regulates specific Ca2+ channel targets in the brain and retina. Multiple isoforms of CaBPs are localized in different neuronal cell types and perform specialized roles in sensory transduction and disease processes1.CaBP1-5 proteins have four EF hands that form pairs within the N lobe (EF1 and 2) and C lobe (EF3 and 4). The two lobes are structurally independent and connected by a flexible linker. Whereas all four EF hands bind Ca2+ in CaM, EF2 in CaBP1 does not bind Ca2+, and EF1 has reduced selectivity for Ca2+ over Mg2+. EF3 and EF4 in the C lobe of CaBP1 exhibit canonical Ca2+-induced conformational changes2. CaBP-target interactions induce functional changes distinct from those caused by CaM and may diversify neuronal responses to Ca2+ signals. CaBPs interact with and reshape the functional properties of certain voltage-gated Ca2+ channels (CaVs)3.CaBP1 is the best characterized family member and has been shown to regulate inositol 1,4,5-triphosphate receptors (IP3Rs), P/Q-type voltage-gated Ca2+ channels, L-type Ca2+ channels, and the transient receptor potential channel (TRPC5)4. CaBP1 regulates the currents of CaV1 and CaV2 channels in neurons, photoreceptors, and auditory hair cells. CaBP interactions with CaV1 channels may be required for hearing and vision, as mutations that disrupt these interactions cause blindness and deafness5.

Gene ID: 171051

UniProt: O88751

Pathways: Synaptic Membrane, Negative Regulation of Transporter Activity