CASR antibody (Extracellular, N-Term)

Catalog No. ABIN7884839

The Rabbit Polyclonal anti-CASR antibody (ABIN7884839) specifically detects CASR in WB, IHC, IF, FACS, LCI and IC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for CASR antibody (Extracellular, N-Term) (ABIN7884839)

Target: CASR (Calcium-Sensing Receptor (CASR))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CASR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Live Cell Imaging (LCI), Immunochromatography (IC)

Grade: Carrier-free, KO Validated

Product Details anti-CASR Antibody

Binding Specificity: AA 216-229, Extracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to Calcium Sensing Receptor

Predicted Reactivity: Rat,mouse,bovine,canis - identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)DDYG RPGIE KFREE, corresponding to amino acid residues 216-229 of human CaSR

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 2.5 μg per reaction

ICC: 1:50

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235016)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for CASR

Target: CASR (Calcium-Sensing Receptor (CASR))

Alternative Name: CaSR

Background:

Synonyms: CaSR, Extracellular calcium-sensing receptor, Parathyroid cell calcium-sensing receptor 1, PCaR1, GPRC2A

Description: The Calcium-sensing receptor (CaSR) is a member of subfamily C of the G-protein-coupled receptor (GPCR) superfamily. As its primary role, the CaSR is a key player in calcium homeostasis and is expressed in tissues involved in calcium metabolism such as parathyroid cells and the kidneys.1,2The expression of CaSR was also described in other cell types and tissues, such as neurons, keratinocytes, and the pancreas. However, the role of CaSR in these cells remains to be established.1,2 Following the identification of functional vitamin D response elements in the CaSR gene, it was suggested that vitamin D might regulate the expression of CaSR.3CaSR activation is followed by a rapid, transient increase in the cytosolic calcium concentration, resulting from mobilization of calcium from thapsigargin-sensitive intracellular stores, as well as an increased calcium influx through voltage-insensitive calcium channels located at the cell membrane.4CaSR regulates cellular processes such as proliferation, apoptosis, and differentiation under both normal and pathologic conditions.5Recent studies demonstrated the expression in human colon epithelium of CaSR, which regulates proliferation and differentiation. In colon carcinomas, lower levels of expression were found in the cancerous tissue compared to normal colon tissue.

Gene ID: 846

UniProt: P41180

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis