CLCN2 antibody (C-Term, Intracellular)

Catalog No. ABIN7884873

This Rabbit Polyclonal antibody specifically detects CLCN2 in WB, IHC, IF and IC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CLCN2 antibody (C-Term, Intracellular) (ABIN7884873)

Target: CLCN2 (Chloride Channel 2 (CLCN2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CLCN2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Grade: Carrier-free, KO Validated

Product Details anti-CLCN2 Antibody

Binding Specificity: AA 888-906, C-Term, Intracellular

Purpose: A Rabbit Polyclonal Antibody to CLC-2 (CLCN2) Channel

Predicted Reactivity: human - 17, guinea pig - 15,swine - 18,19 amino acid residues identical,Mouse - identical, rabbit

Purification: Affinity purified on immobilized antigen.

Immunogen: RSRHGLPREGTPSDSDDKC, corresponding to amino acid residues 888-906 of rat CLC-2.

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235082)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for CLCN2

Target: CLCN2 (Chloride Channel 2 (CLCN2))

Alternative Name: Chloride channel 2

Background:

Synonyms: Chloride channel 2, ClC2

Description: CLC-2 is a member of the voltage-dependent Cl- channel (CLC) family that includes nine known members in mammals. CLC channels can be classified as plasma membrane channels and intracellular organelle channels. The first group includes the CLC-1, CLC-2 CLC-Ka and CLCKb channels. The second group comprises the CLC-3, CLC-4, CLC-5, CLC-6 and CLC-7.CLC channels that function in the plasma membrane are involved in the stabilization of membrane potential and in transepithelial transport. The presumed function of the intracellular CLC channels is support of the acidification of the intraorganellar compartment. In this regard, recent reports indicate that ClC-4 and ClC-5 (and by inference ClC-3) can function as Cl-/H+ antiporters.1, 2The functional unit of the CLC channels is a dimer with each subunit forming a proper pore. Although the crystal structure of bacterial CLC channels was resolved, the topology of the CLC channels is complex and has not been fully elucidated. It is generally accepted that both the N- and C- terminus domains are intracellular while the number and configuration of the transmembrane domains vary greatly between different models. 1,2CLC-2 is widely distributed with prominent expression in brain, kidney, lung and the gastrointestinal system. Mutations in the CLC-2 channel gene detected in humans are associated with idiopathic generalized epilepsies while disruption of the ClC-2 gene in mice is associated with testicular and retinal degeneration. 3,4

Gene ID: 29232

UniProt: P35525