CNTF antibody (C-Term)

Catalog No. ABIN7884883

The Rabbit Polyclonal anti-CNTF antibody is suitable to detect CNTF in samples from Human, Rat and Mouse. It has been validated for WB, IHC, IF and IC.

Quick Overview for CNTF antibody (C-Term) (ABIN7884883)

Target: CNTF (Ciliary Neurotrophic Factor (CNTF))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CNTF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Grade: Carrier-free

Product Details anti-CNTF Antibody

Binding Specificity: AA 164-177, C-Term

Purpose: A Rabbit Polyclonal Antibody to CNTF

Predicted Reactivity: Rat,mouse - identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)ELSQWTVRSIHDLR, corresponding to amino acid residues 164-177 of human CNTF

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: 1:200

IHC: 1:200

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235138)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for CNTF

Target: CNTF (Ciliary Neurotrophic Factor (CNTF))

Alternative Name: Ciliary neurotrophic factor

Background:

Synonyms: Ciliary neurotrophic factor

Description: CNTF is a polypeptide trophic factor, member of the alpha-helical cytokine superfamily1. It was initially purified from the chick eye on the basis of its ability to promote survival of E8 chick ciliary ganglion neurons in culture2. CNTF is synthesized by glia both in the CNS and PNS3 and it has been demonstrated that CNTF is ubiquitously distributed in neurons and glia throughout the rodent brain4. CNTF effects are mediated by a tripartite receptor complex consisting of two signal-transducing subunits (leukemia inhibitory factor receptor, gp130) and a CNTF-specific ligand-binding-subunit (CNTFR)5.CNTF can support the survival of many different cell populations within the PNS and the CNS6. in vitro, CNTF promotes proliferation and neuronal specifications in hippocampal neurons. in vivo, it supports the viability of non-primate motor neurons7, induces sprouting of cholinergic motor neurons8 and delays neural degeneration in genetic models of motor neuron disease9. In addition, it is involved in the development stage of astrocytes and oligodendocytes10.

Gene ID: 1270

UniProt: P26441

Pathways: JAK-STAT Signaling