CNTF Receptor alpha antibody (Extracellular)

Catalog No. ABIN7884884

The Rabbit Polyclonal anti-CNTF Receptor alpha antibody (ABIN7884884) specifically detects CNTF Receptor alpha in WB, IHC, IF, FACS and LCI. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for CNTF Receptor alpha antibody (Extracellular) (ABIN7884884)

Target: CNTF Receptor alpha (CNTFR) (Ciliary Neurotrophic Factor Receptor (CNTFR))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CNTF Receptor alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Grade: Carrier-free

Product Details anti-CNTF Receptor alpha Antibody

Binding Specificity: AA 164-178, Extracellular

Purpose: A Rabbit Polyclonal Antibody to CNTF Receptor α

Predicted Reactivity: Mouse,human - identical

Purification: Affinity purified on immobilized antigen.

Immunogen: CHIRYMHLFSTIKYK, corresponding to amino acid residues 164-178 of rat CNTFRalpha

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:400

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235141)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for CNTF Receptor alpha

Target: CNTF Receptor alpha (CNTFR) (Ciliary Neurotrophic Factor Receptor (CNTFR))

Alternative Name: Ciliary neurotrophic factor receptor subunit alpha

Background:

Synonyms: Ciliary neurotrophic factor receptor subunit alpha, CNTF receptor alpha, CNTFR alpha

Description: CNTF is a polypeptide trophic factor, member of the alpha-helical cytokine superfamily1. It was initially purified from the chick eye on the basis of its ability to promote survival of E8 chick ciliary ganglion neurons in culture2. CNTF is synthesized by glia both in the CNS and PNS3 and it has been demonstrated that CNTF is ubiquitously distributed in neurons and glia throughout the rodent brain4. CNTF effects are mediated by a tripartite receptor complex consisting of two signal-transducing subunits (leukemia inhibitory factor receptor, gp130) and a CNTF-specific ligand-binding-subunit (CNTFRα)5.CNTFRα is anchored to the membrane by a glycosyl-phosphatidylinositol (GPI) linkage and lacks the intracellular signaling moiety. It can be cleaved from the GPI anchor to yield a soluble form of the receptor (sCNTFRα)6,7.CNTF binds to CNTFRα, the latter associates with gp130 which recruits LIFR. This leads to the activation of a signaling cascade involving the JAK/STAT pathway7.CNTFRα knockout mice die within 24 hours after birth as opposed to CNTF knock animals which are viable. This strongly suggest that CNTFRα has other ligands besides CNTF. Indeed, CLC/CLF complex (cardiotrophin like cytokine/cytokine-like factor-1) binds CNTFRα and activates a similar signaling pathway to that of CNTF8.

Gene ID: 313173

UniProt: Q08406

Pathways: JAK-STAT Signaling, Feeding Behaviour