Ephrin B1 antibody (Extracellular Domain)

Catalog No. ABIN7884945

This Rabbit Polyclonal antibody specifically detects Ephrin B1 in WB, FACS and LCI. It exhibits reactivity toward Human and Rat.

Quick Overview for Ephrin B1 antibody (Extracellular Domain) (ABIN7884945)

Target: Ephrin B1 (EFNB1)

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ephrin B1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Grade: Carrier-free

Product Details anti-Ephrin B1 Antibody

Binding Specificity: AA 208-221, Extracellular Domain

Purpose: A Rabbit Polyclonal Antibody to Ephrin-B1

Predicted Reactivity: Mouse - identical, rat - 13,14 amino acid residues identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)KHETVNQEEKSGPG, corresponding to amino acid residues 208-221 of human Ephrin-B1

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235313)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Ephrin B1

Target: Ephrin B1 (EFNB1)

Alternative Name: EPH-related receptor tyrosine kinase ligand 2

Background:

Synonyms: EPH-related receptor tyrosine kinase ligand 2, LERK2, EFNB1

Description: The erythropoietin-producing hepatocellular (EPH) receptors represent the largest known family of receptor tyrosine kinases and are activated by interactions with cell-surface ligands, termed EFNs. EPH receptors have been classified into two subfamilies, EPHA and EPHB, according to their preference for either glycosylphosphatidylinositol (GPI)-anchored EFN-A ligands or transmembrane EFN-B ligands. Thus far, 14 vertebrate receptors and eight ligands have been identified1. All the ligands share a conserved core sequence of approximately 125 amino acids, including 4 invariant cysteine residues, probably corresponding to a receptor binding domain. This is followed by a membrane anchorage domain, taking the form of a GPI anchor for five of the ligands (ephrin-A1 to -A5) or a transmembrane domain for three of them (ephrin-B1 to -B3) 2.Ephrin-B1 is signals forward or reverse signaling mechanisms. Ephrin-B1 PDZ-dependent reverse signaling controls axon guidance of the corpus callosum, whereas forward signaling is critical for normal craniofacial development2.Eph receptors and ephrins are highly expressed in the brain and in the developing nervous system, where they are involved in fundamental developmental processes of the nervous system, including axon guidance, axonfasciculation, neural crest cell migration, acquisition of brain subregional identity,and neuronal cell survival. Ephrins are dramatically expressed in a wide range of regions of the vertebrate embryo including the ectoderm, mesoderm, and endoderm3.Overexpression of B-type ephrin in cancer cells is reported to correlate with high invasion and high vascularity of tumors and elevated expression of ephrin-B1 is observed in poorly differentiated invasive tumor cells and other tumors with poor clinical prognosis4.

Gene ID: 1947

UniProt: P98172

Pathways: RTK Signaling