GALR2 antibody (3rd Intracellular Loop)

Catalog No. ABIN7885009

The Rabbit Polyclonal anti-GALR2 antibody is suitable to detect GALR2 in samples from Human, Mouse and Rat. It has been validated for WB, IHC and IF.

Quick Overview for GALR2 antibody (3rd Intracellular Loop) (ABIN7885009)

Target: GALR2 (Galanin Receptor 2 (GALR2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GALR2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Grade: Carrier-free

Product Details anti-GALR2 Antibody

Binding Specificity: 3rd Intracellular Loop, AA 223-235

Purpose: A Rabbit Polyclonal Antibody to Galanin Receptor Type 2

Predicted Reactivity: Rat - identical, human - 11,13 amino acid residues identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)GSGSQRAKRKVTR, corresponding to amino acid residues 223-235 of mouse Galanin receptor type 2

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:100

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235477)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for GALR2

Target: GALR2 (Galanin Receptor 2 (GALR2))

Alternative Name: Galanin receptor type 2

Background:

Synonyms: Galanin receptor type 2, GALNR2, GAL2R

Description: Galanin is a neuropeptide, consisting of 29-30 amino acid residues, involved in the regulation of cognition, mood, feeding, seizure activity and pain. Galanin exerts its action via three known receptors, the Galanin receptor type 1 (GALR1), Galanin receptor type 2 (GALR2) and Galanin receptor type 3 (GALR3). The Galanin receptors belong to the G-protein coupled receptor superfamily.1-4GALR2 is widely expressed in the brain (with highest levels of mRNA found in the hypothalamus, hippocampus, amygdala and pyriform cortex) and in peripheral tissues such as the small intestine, heart, kidney, and liver. High levels of GALR2 expression were also found in rat dorsal root ganglia.1GALR2 activation increases inositol phosphate hydrolysis, mediates the release of Ca2+ into the cytoplasm from intracellular stores, and opens Ca2+-dependent chloride channels in a PTX-resistant manner, indicating that GALR2 couples to Gq/11 proteins.5

Gene ID: 14428

UniProt: O88854

Pathways: cAMP Metabolic Process, Inositol Metabolic Process, Feeding Behaviour