GRPR antibody (3rd Extracellular Loop)

Catalog No. ABIN7885033

The Rabbit Polyclonal anti-GRPR antibody is suitable to detect GRPR in samples from Human, Mouse and Rat. It has been validated for WB, IHC, IF, IC and LCI.

Quick Overview for GRPR antibody (3rd Extracellular Loop) (ABIN7885033)

Target: GRPR (Gastrin-Releasing Peptide Receptor (GRPR))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRPR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)

Grade: Carrier-free

Product Details anti-GRPR Antibody

Binding Specificity: 3rd Extracellular Loop, AA 287-300

Purpose: A Rabbit Polyclonal Antibody to Bombesin Receptor 2/Gastrin-releasing Peptide Receptor

Predicted Reactivity: Rat,mouse,dog - identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)RSYHYSEVDTSMLH, corresponding to amino acid residues 287-300 of human BB2R 

Isotype: IgG

Application Details

Application Notes:

WB: 1:200-1:500

FC: The optimal concentration should be determined by the user

ICC: 1:100

IHC: 1:50

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235573)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for GRPR

Target: GRPR (Gastrin-Releasing Peptide Receptor (GRPR))

Alternative Name: BB2R

Background:

Synonyms: BB2R, Gastrin-releasing peptide receptor, GRP-preferring bombesin receptor

Description: Bombesin receptor 2 (BB2) is a member of a family of receptors that binds the 14 amino acid peptide, bombesin. Bombesin was first isolated from frog skin and it was later established that mammals express endogenous bombesin-like peptides such as gastrin-releasing peptide (GRP, a 27 amino acid homologue) and neuromedin B (NMB, a 10 amino acid homologue).BB2 is the preferred receptor for GRP and hence it is also referred to as GRP receptor or GRP-R.1 BB1 is the preferred receptor for NMB and therefore it is also known as NMB-R. A third member (called BB3) of the bombesin receptor family has been recognized but the endogenous mammalian ligand for this receptor remains to be identified.All three bombesin receptors are members of the seven-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. BB2 is coupled to a Gq/11 protein that activates phospholipase C (PLC) and leads to production of inositol 1,4,5-trisphosphate (InsP3), intracellular Ca2+ mobilization, and cell growth.2GRP (through its binding to BB2) plays an essential role in the release of hormones in the intestinal tract, the regulation of smooth muscle contraction, and the secretion of pancreatic enzymes.2 In the brain, BB2 has been implicated in regulating feeding behavior, thermoregulation, and memory formation.3Finally, BB2 is overexpressed in several human tumors including breast, prostate, and lung, where it acts as a growth factor receptor inducing tumor growth. BB2 is considered a potential target for the development of both diagnostics and anti-cancer therapies.4

Gene ID: 2925

UniProt: P30550