Gephyrin antibody (Intracellular)

Catalog No. ABIN7885038

This Rabbit Polyclonal antibody specifically detects Gephyrin in WB, IHC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Gephyrin antibody (Intracellular) (ABIN7885038)

Target: Gephyrin (GPHN)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Gephyrin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Grade: Carrier-free

Product Details anti-Gephyrin Antibody

Binding Specificity: AA 183-195, Intracellular

Purpose: A Rabbit Polyclonal Antibody to Gephyrin

Predicted Reactivity: Mouse,human - identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)RDAIVKVKEVHDE, corresponding to amino acid residues 183-195 of rat Gephyrin

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235588)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Gephyrin

Target: Gephyrin (GPHN)

Alternative Name: GPHN

Background:

Synonyms: GPHN, Putative glycine receptor-tubulin linker protein, MPT adenylyltransferase, MPT Mo-transferase

Description: Gephyrin is a highly conserved, intracellular scaffolding protein responsible for the post-synaptic anchoring and assembly of the inhibitory glycine and γ-aminobutyric acid type A receptors (GlyR and GABAAR), it essentially anchors the receptors, by auto-oligomerization, to the cytoskeleton1,2.GABAergic transmission is important for the mediation of neuronal excitability and has a crucial role in neurodevelopment, throughout the central and peripheral nervous systems (especially via GABAAR). Glycinergic neurons on the other hand control motor functions and sensory signaling as well as take part in pain transmission in the spine's dorsal horn. Importantly, gephyrin has a 1:1 stoichiometry with GlyR3,4.Up-to-date atomic force microscopy data of gephyrin has shown that it forms trimers with multiple conformations. It is a modular construct with three domains: an N-terminal G-, a central C- and a C-terminal E-domain. Recent work suggests also that gephyrin regulates neuronal differentiation by anchoring small cytoskeletal-related signaling molecules at unique subcellular locations2,5,6.

Gene ID: 64845

UniProt: Q03555

Pathways: Synaptic Membrane, Skeletal Muscle Fiber Development