Metabotropic Glutamate Receptor 3 antibody (Extracellular, N-Term)

Catalog No. ABIN7885061

The Rabbit Polyclonal anti-Metabotropic Glutamate Receptor 3 antibody (ABIN7885061) specifically detects Metabotropic Glutamate Receptor 3 in WB, IHC, IF, IC and LCI. The antibody is reactive with Mouse and Rat samples.

Quick Overview for Metabotropic Glutamate Receptor 3 antibody (Extracellular, N-Term) (ABIN7885061)

Target: Metabotropic Glutamate Receptor 3 (GRM3) (Glutamate Receptor, Metabotropic 3 (GRM3))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Metabotropic Glutamate Receptor 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)

Grade: Carrier-free, KO Validated

Product Details anti-Metabotropic Glutamate Receptor 3 Antibody

Binding Specificity: Extracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to Metabotropic Glutamate Receptor 3

Predicted Reactivity: Mouse - identical, human,monkey - 14,15 amino residues identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)GDHNFMRREIKIEGD, corresponding to amino acids 24-38 of rat mGluR3

Isotype: IgG

Application Details

Application Notes:

WB: 1:600

FC: The optimal concentration should be determined by the user

ICC: 1:50

IHC: 1:100

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235689)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Metabotropic Glutamate Receptor 3

Target: Metabotropic Glutamate Receptor 3 (GRM3) (Glutamate Receptor, Metabotropic 3 (GRM3))

Alternative Name: Metabotropic glutamate receptor 3

Background:

Synonyms: Metabotropic glutamate receptor 3, GRM3, mGlu3, GPRC1C

Description: L-glutamate, the major excitatory neurotransmitter in the central nervous system, operates through several receptors that are categorized as ionotropic (ligand-gated cation channels) or metabotropic (G-protein coupled receptors). The metabotropic glutamate receptor family includes eight members (mGluR1-8) that have been divided into three groups based on their sequence homology, pharmacology and signal transduction.Group II of the metabotropic glutamate receptors includes the mGluR2 and mGluR3 receptors. The receptors present the typical G-protein coupled receptor (GPCR) signature topology: seven transmembrane domains with a large extracellular N-terminus that contains the glutamate binding site, and an intracellular C-terminus.1,2 mGluR2 and mGluR3 are coupled to Gi/G0 and hence inhibit cAMP formation following receptor activation.1,2mGluR3 is expressed in neurons and glia in many brain regions including the cerebral cortex, hippocampus and brain stem. mGluR3 expression is predominantly presynaptic consistent with its role as an inhibitory autoreceptor, that is, activation of mGluR3 by glutamate inhibits additional glutamate release from the same neurons.1,2Several lines of evidence suggest that mGluR3 is important for long term depression, glial function and neuroprotection. Some studies, though not all, have shown a genetic association of mGluR3 gene polymorphisms with psychosis and with schizophrenia-related phenotypes. Based in these findings, mGluR3 has been suggested as a therapeutic target for both psychosis and schizophrenia.3

Gene ID: 24416

UniProt: P31422

Pathways: cAMP Metabolic Process, Synaptic Membrane