Mesothelin antibody (Extracellular, Loop 3) (APC)

Catalog No. ABIN7885140

This Rabbit Polyclonal antibody specifically detects Mesothelin in FACS and LCI. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Mesothelin antibody (Extracellular, Loop 3) (APC) (ABIN7885140)

Target: Mesothelin (MSLN)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Mesothelin antibody is conjugated to APC

Application: Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-Mesothelin Antibody (APC)

Binding Specificity: AA 294-307, Extracellular, Loop 3

Purpose: A Rabbit Polyclonal Antibody to GPER1 (extracellular) conjugated to Allophycocyanin (APC) fluorophore.

Predicted Reactivity: Rat - identical, human - 13,14 amino acid residues identical.

Purification: Affinity purified on immobilized antigen.

Immunogen: Peptide CKQSFRHAYPLTGH, corresponding to amino acid residues 294 - 307 of mouse GPER1

Isotype: IgG

Application Details

Application Notes: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7582043)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 15 μL or 50 μL double distilled water (DDW), depending on the sample size.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Mesothelin

Target: Mesothelin (MSLN)

Alternative Name: GPER1

Background:

Synonyms: G Protein-Coupled Estrogen Receptor 1, G Protein-Coupled Receptor 30, Membrane Estrogen Receptor, Chemoattractant Receptor-Like 2, GPER

Description:

GPER1/GPR30 is an estrogen receptor belonging to the superfamily of G-protein coupled receptors (GPCRs). Like all members, GPER1 has seven membrane-spanning domains, an extracellular N-terminus and a cytoplasmic C-terminal domain. It is widely expressed in the central nervous system (CNS) and it binds 17β-estradiol (17β-E) with high affinity1.

Estrogens have long been known as important regulators of the female reproductive functions, however, is now commonly accepted that estrogens and androgens have important functions in both female and male physiology and pathology.

As opposed to the epigenetic regulatory role in estrogen signaling carried out by ER-α and ER-β estrogen receptors, signaling from GPER1 is responsible for the rapid non-genomic actions of estrogens that activate various protein-kinase cascades1. GPER1 signaling improves spatial memory, possibly via release of neurotransmitters. In addition, GPER1 activation contributes to behaviors that denote anxiety, and to social behaviors such as social memory and lordosis behavior in mice. In the male hippocampus, GPER1 activation leads to phosphorylation of the classical estrogen receptor ERα, suggesting that crosstalk with ERα is important in the manifestation of these behaviors, many of which are absent in ERα-null mice2. GPER1 activation also has several beneficial effects in the cardiovascular system including vasorelaxation, inhibition of smooth muscle cell proliferation, and protection of the myocardium against ischemia/reperfusion injury, and in the metabolic system including stimulation of insulin release and protection against pancreatic β-cell apoptosis. Thus, GPER1 is emerging as a candidate therapeutic target in both cardiovascular and metabolic diseases3.

Gene ID: 76854

UniProt: Q8BMP4

Pathways: EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process