NPTN antibody (Extracellular, N-Term)

Catalog No. ABIN7885193

The Rabbit Polyclonal anti-NPTN antibody (ABIN7885193) specifically detects NPTN in WB, IHC, IF, FACS and LCI. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for NPTN antibody (Extracellular, N-Term) (ABIN7885193)

Target: NPTN (Neuroplastin (NPTN))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NPTN antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Grade: Carrier-free

Product Details anti-NPTN Antibody

Binding Specificity: AA 194-210, Extracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to Neuroplastin

Predicted Reactivity: Mouse,human - identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)RKNASNMEYRINKPRAE, corresponding to amino acid residues 194-210 of rat NPTN

Isotype: IgG

Application Details

Application Notes:

WB: 1:400

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:120

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7236121)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for NPTN

Target: NPTN (Neuroplastin (NPTN))

Alternative Name: NPTN

Background:

Synonyms: NPTN, Glycoprotein 55/65, GP55, GP65, Np55, Np65, Stromal cell-derived receptor 1, SDR1, SDFR1

Description: The neuroplastins Np55 and Np65 comprise a superfamily of neuronal and synapse enriched immunoglobulin molecules. They play an important role in a number of vital synaptic and neuronal activities including cell adhesion in the case of Np65. Np65 and Np55 are identical in structure except for the N-terminal Np65-specific Ig1 domain. They also play a key role in the localization of GABA(A) receptors in inhibitory synapses.Neuroplastins have two Ig domains that project into the extracellular space and are followed by a single 21 amino-acid membrane spanning sequence and a 34 amino-acid intracellular domain. The intracellular domain is mostly comprised of charged and neutral residues but also of a few hydrophobic residues. The N-terminus includes a 28 amino-acid signal peptide. In addition, neuroplastins contain a glutamate residue in their transmembrane domain which is important for molecular interactions within the membrane region.Although Np55 and Np65 are closely related in structure there are significant differences in terms of their expression. Np65 is tissue specific and is expressed mostly in forebrain neurons in the cortex, hippocampus and striatum. It has an anterior-posterior axis of expression. It is also expressed in lower amounts in the thalamus and hypothalamus. In contrast, Np55 is expressed throughout the brain in varying amounts.Currently, neuroplastins are not directly implicated in human diseases however several possible links have been discovered. A single nucleotide polymorphism (SNP) in the human neuroplastin locus is associated with reduced cortical thickness and impaired intellectual ability in adolescents. Furthermore, other SNPs in neuroplastin has been found to be either a risk or protecting factor for schizophrenia in animal models1.

Gene ID: 56064

UniProt: P97546

Pathways: Regulation of long-term Neuronal Synaptic Plasticity