Neurotrophin 3 antibody (Intracellular)

Catalog No. ABIN7885199

The Rabbit Polyclonal anti-Neurotrophin 3 antibody (ABIN7885199) specifically detects Neurotrophin 3 in WB, IHC and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Neurotrophin 3 antibody (Intracellular) (ABIN7885199)

Target: Neurotrophin 3 (NTF3)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Neurotrophin 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Grade: Carrier-free

Product Details anti-Neurotrophin 3 Antibody

Binding Specificity: AA 39-48, AA 177-186, Intracellular

Purpose: A Rabbit Polyclonal Antibody to Neurotrophin 3

Predicted Reactivity: Rat,mouse,chicken,Xenopus - identical, cat - 9,10 amino acid residues identical

Purification: Affinity purified on immobilized antigen.

Immunogen: GEIKTGNSPV(C), corresponding to amino acid residues 39-48 of mature human NT-3 (residues 177-186 of the NT-3 precursor)

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:300

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7236144)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Neurotrophin 3

Target: Neurotrophin 3 (NTF3)

Alternative Name: NTF3

Background:

Synonyms: NTF3, Nerve growth factor 2, NGF2, HDNF

Description: The neurotrophins ("neuro" meaning nerve and "trophe" meaning nutrient) are a family of soluble, basic growth factors which regulate neuronal development, maintenance, survival and death in the CNS and PNS.1 The structural hallmark of all neurotrophins is the characteristic arrangement of the disulfide bridges, known as the cysteine knot, which has been found in other growth factors such as PDGF.2 The rat and human forms of neurotrophin-3 (NT-3) are 96 % homologous. NT-3 has been shown to strengthen synaptic connections to motoneurons in the neonatal rat,3 to serve as an anti-inflammatory factor, to suppress microglial activation,4 to play a critical role in regulating T helper 1/T helper 2 cell balance5 and to modify potassium currents in isolated inner hair cells from guinea pig cochlea.6 The biological effects of NT-3 are mediated by two receptors: TrkC, which is specific for NT-3, and p75NTR, which binds all the neurotrophins.7

Gene ID: 4908

UniProt: P20783

Pathways: RTK Signaling, Neurotrophin Signaling Pathway