OPRL1 antibody (C-Term, Intracellular)

Catalog No. ABIN7885207

The Rabbit Polyclonal anti-OPRL1 antibody is suitable to detect OPRL1 in samples from Human, Mouse and Rat. It has been validated for WB.

Quick Overview for OPRL1 antibody (C-Term, Intracellular) (ABIN7885207)

Target: OPRL1 (Opiate Receptor-Like 1 (OPRL1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OPRL1 antibody is un-conjugated

Application: Western Blotting (WB)

Grade: Carrier-free

Product Details anti-OPRL1 Antibody

Binding Specificity: AA 337-352, C-Term, Intracellular

Purpose: A Rabbit Polyclonal Antibody to Nociceptin Receptor

Predicted Reactivity: Mouse - identical, human - 13,16 amino acid residues identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)HREMQVSDRVRSIAKD, corresponding to amino acid residues 337-352 of rat OPRL1

Isotype: IgG

Application Details

Application Notes:

WB: 1:600

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7236167)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for OPRL1

Target: OPRL1 (Opiate Receptor-Like 1 (OPRL1))

Alternative Name: kappa-Type 3 opioid receptor

Background:

Synonyms: κ-Type 3 opioid receptor, KOR-3, Orphanin FQ receptor, ROR-C, XOR1, ORL1, N/OFQ receptor

Description: Nociceptin/orphanin FQ (N/OFQ), a 17 amino acid peptide, is the endogenous agonist of the nociceptin/orphanin FQ peptide (NOP) receptor (previously termed ORL1 receptor) which belongs to the class A (Rhodopsin-like) γ subfamily of G protein-coupled receptors (GPCRs). Like all members the receptor has a common seven-transmembrane helical structure and is coupled predominantly to heterotrimeric Gi/Go proteins. N/OFQ receptor shows a high degree of structural homology with the μ-, δ- and κ-opioid receptors1. The intracellular events triggered by the binding of N/OFQ with the NOP receptor include inhibition of adenylyl cyclase, activation of phospholipase C (PLC) and of K+ channels, inhibition of Ca2+ channels, and activation of mitogen-activated protein-kinases (MAPKs). In neural tissues, the main effect is the inhibition of the release of neurotransmitters (acetylcholine, catecholamines, GABA, glutamate, 5-hydroxytryptamine, tachykinines)2.NOP receptor mRNA is detected in high levels in the cortex, anterior olfactory nucleus, lateral septum, hypothalamus, hippocampus, amygdala, central grey, pontine nuclei, interpeduncular nucleus, substantia nigra, raphe complex, locus coeruleus and spinal cord3.Studies with N/OFQ, NOP-selective agonists or antagonists, and receptor- or peptide-deficient mice have shown that the NOP system has important roles in the control of central and peripheral functions including pain, anxiety depression, hyperphagia and obesity, addiction, Parkinson's disease, cognition, food intake, learning and memory, locomotion, cough and micturition reflexes, cardiovascular homeostasis, intestinal motility and immune responses4.

Gene ID: 29256

UniProt: P35370