Peroxisomal Biogenesis Factor 5-Like (PEX5L) (AA 151-165), (Intracellular) antibody

Catalog No. ABIN7885227

This Rabbit Polyclonal antibody specifically detects Peroxisomal Biogenesis Factor 5-Like in IHC, WB and IF. It exhibits reactivity toward Rat and Mouse.

Quick Overview for Peroxisomal Biogenesis Factor 5-Like (PEX5L) (AA 151-165), (Intracellular) antibody (ABIN7885227)

Target: Peroxisomal Biogenesis Factor 5-Like (PEX5L)

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)

Grade: Carrier-free, KO Validated

Product Details

Binding Specificity: AA 151-165, Intracellular

Purpose: A Rabbit Polyclonal Antibody to PEX5L (TRIP8b)

Predicted Reactivity: rabbit - 14, human - 13,15 amino acid residues identical,Mouse - identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)EKWDDVKFHGDRTSK, corresponding to amino acid residues 151-165 of rat PEX5-related protein

Isotype: IgG

Application Details

Application Notes:

WB: 1:400

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:300

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7236249)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details

Target: Peroxisomal Biogenesis Factor 5-Like (PEX5L)

Alternative Name: PEX5R

Background:

Synonyms: PEX5R, PEX5Rp, PEX5-related protein, PEX5-like protein, Peroxin-5-related protein, PEX2-related protein, PXR2, Peroxisome biogenesis factor 5-like, TPR-containing Rab8b-interacting protein

Description: PEX5R (also called TRIP8b, tetratricopetide repeat-containing Rab8b-interacting protein) is a cytoplasmic protein expressed as a family of alternatively spliced isoforms. PEX5R /TRIP8b isoforms contain a large constant domain preceded by a variable region. The C-terminal half of TRIP8b comprises a tetratricopeptide repeat (TPR) protein binding domain1. Analysis of the Pex5p-like protein revealed the presence of a tetratricopeptide repeat (TPR) domain in its C-terminal half consisting of seven TPR motifs. Pex5R is almost exclusively expressed in brain2.Recent studies provide strong evidence that PEX5R interacts with the carboxyl-terminal region of Hyperpolarization-activated cyclic nucleotide-gated (HCN) channels and regulates their cell-surface expression level and cyclic nucleotide dependence3,4. HCN channels are key modulators of neuronal activity by providing the depolarizing cation current involved in rhythmogenesis, dendritic integration, and synaptic transmission. In contrast, Gosh et al. have shown that human PEX5R can bind to peroxisomal targeting signal-1 (PTS1) ligands5.Furthermore it was shown that enzyme alanine-glyoxylate aminotransferase (AGT) is recognized by PEX5R in the cytoplasm, which allows its subsequent translocation into the peroxisome6. TRIP8b may play a role in both normal neuronal function and in aberrant neuronal excitability associated with neurological diseases7.

Gene ID: 286937

UniProt: Q925N3

Pathways: Positive Regulation of Peptide Hormone Secretion, Maintenance of Protein Location