Prokineticin Receptor 2 antibody (Extracellular, N-Term)

Catalog No. ABIN7885322

The Rabbit Polyclonal anti-Prokineticin Receptor 2 antibody (ABIN7885322) specifically detects Prokineticin Receptor 2 in WB, IHC, FACS, IF and LCI. The antibody is reactive with Mouse and Rat samples.

Quick Overview for Prokineticin Receptor 2 antibody (Extracellular, N-Term) (ABIN7885322)

Target: Prokineticin Receptor 2 (PROKR2)

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Prokineticin Receptor 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Live Cell Imaging (LCI)

Grade: Carrier-free, KO Validated

Product Details anti-Prokineticin Receptor 2 Antibody

Binding Specificity: AA 3-15, Extracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to Prokineticin Receptor 2

Predicted Reactivity: Mouse - 11,13 amino acid residues identical, human - 9

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)DQNGNTSFAPDLN, corresponding to amino acid residues 3-15 of rat PROKR2

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:100

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7236613)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Prokineticin Receptor 2

Target: Prokineticin Receptor 2 (PROKR2)

Alternative Name: PROKR2

Background:

Synonyms: PROKR2, PKR2, G-protein coupled receptor 73-like 1, Gpr73l1, Gpr73b, G-protein coupled receptor I5E

Description: The Prokineticins (PK1 and PK2) are a pair of cysteine-rich secreted peptides with broad physiological functions including gastrointestinal motility, angiogenesis, hematopoiesis and circadian rhythms regulation.The biological effects of PK1 and PK2 are mediated by two highly homologous receptors termed Prokineticin receptor 1 (PKR1) and Prokineticin receptor 2 (PKR2) that belong to the 7-transmembrane domain, G-protein coupled receptor (GPCR) superfamily. Both PK ligands activate the two PK receptors with similar potency.1PKR2 receptors couple to Gq/G11 proteins leading to phospholipase C activation, inositol phosphate production and calcium mobilization.The distribution of PKR2 is relatively restricted with high expression levels in the brain, spinal cord and dorsal root ganglions, in organs of the reproductive system and in endocrine tissues such as the thyroid, pituitary and adrenal glands.1The PK2 ligand has been shown to be involved in the regulation of circadian rhythms of physiological and behavioral processes in mammals, probably through signaling via PKR2 which is highly expressed in the suprachiasmatic nucleus (SCN), an area of the brain that controls circadian rhythm processes.2In addition, loss-of-function mutations in the PKR2 gene have been associated with Kallmann Syndrome, a condition characterized by idiopathic hypogonadotropic hypogonadism (IHH) in combination with anosmia, a compromised sense of smell.3

Gene ID: 192649

UniProt: Q8R415

Pathways: Hedgehog Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma