Reticulon 4 antibody (Extracellular)

Catalog No. ABIN7885369

The Rabbit Polyclonal anti-Reticulon 4 antibody is suitable to detect Reticulon 4 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, FACS, IF, IC and LCI.

Quick Overview for Reticulon 4 antibody (Extracellular) (ABIN7885369)

Target: Reticulon 4 (RTN4)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Reticulon 4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)

Grade: Carrier-free

Product Details anti-Reticulon 4 Antibody

Binding Specificity: AA 366-381, Extracellular

Purpose: A Rabbit Polyclonal Antibody to Nogo Receptor

Predicted Reactivity: Mouse,rat - 15,16 amino acid residues identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)GDSPPGNGSGPRHIND corresponding to amino acid residues 366-381 of the human Nogo receptor

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7236730)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Reticulon 4

Target: Reticulon 4 (RTN4)

Alternative Name: NogoR

Background:

Synonyms: NogoR, Nogo-R, NgR, NgR1, Nogo-66 receptor, Reticulon 4 receptor, RTN4R

Description: The Nogo receptor is a leucine-rich repeat (LRR) containing protein with a glycosylphosphatidylinositol (GPI) anchored C-terminus. The receptor was identified on the basis of its ability to bind with high affinity to Nogo-A a member of the reticulon family expressed in the cell membrane of oligodendrocytes.1Nogo-A attracted attention when it was demonstrated that it is a myelin protein capable of inhibiting axonal growth following nerve injury.2,3 Other myelin proteins also identified as axonal growth inhibitors are the myelin-associated glycoprotein (MAG) a sialic-dependent immunoglobulin-like family member lectin (SIGLEC) and the oligodendrocyte myelin glycoprotein (OmgP) which is a GPI-anchored membrane protein. Remarkably, despite their structural diversity, all three axonal growth inhibitors bind to the Nogo receptor with high affinity.4,5Since the Nogo receptor is a GPI-anchored protein it was expected that it would require another protein component to transduce the Nogo-A binding information into the responding neurons interior. Indeed, it was shown that the neurotrophin receptor p75 (p75NTR) interacts with Nogo receptor and a third protein termed LINGO-1 to mediate axonal growth inhibition signaling.6,7

Gene ID: 65078

UniProt: Q9BZR6

Pathways: Neurotrophin Signaling Pathway, Regulation of Cell Size, SARS-CoV-2 Protein Interactome