Serotonin Receptor 3B antibody (Extracellular, N-Term)

Catalog No. ABIN7885393

The Rabbit Polyclonal anti-Serotonin Receptor 3B antibody is suitable to detect Serotonin Receptor 3B in samples from Rat and Mouse. It has been validated for WB, IHC and IF.

Quick Overview for Serotonin Receptor 3B antibody (Extracellular, N-Term) (ABIN7885393)

Target: Serotonin Receptor 3B (HTR3B)

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Serotonin Receptor 3B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Grade: Carrier-free

Product Details anti-Serotonin Receptor 3B Antibody

Binding Specificity: AA 213-226, Extracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 3B

Predicted Reactivity: Mouse - identicalHuman- not recommended for use with human samples

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)HIRQSSAGDFAQIR, corresponding to amino acid residues 213-226 of rat 5-Hydroxytryptamine receptor 3B

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Serotonin Receptor 3B

Target: Serotonin Receptor 3B (HTR3B)

Alternative Name: 5-Hydroxytryptamine receptor 3B

Background:

Synonyms: 5-Hydroxytryptamine receptor 3B, Serotonin receptor 3B

Description: 5-Hydroxytryptamine receptor 3B (5-HT3B, HTR3B) belongs to the superfamily of ligand-gated ion channels. Serotonin receptors, other than the 5-HT3 subtype, belong to the superfamily of G-protein coupled receptors.The 5-HT3 receptor is formed by five subunits arranged around a pore forming unit. Receptors could be either monomeric, such as 5-HT3A or heteromeric entities like 5-HT3A/B. Indeed, the type of channel formed displays different pharmacological and electrophysiological characteristics1,2. To date, five 5-HT3 subunits have been identified and labeled 5-HT3A-E, which show variability in the N-terminus and in the transmembrane region2. 5-HT3A and 5-HT3B are the best characterized among the different types.In general, 5-HT3 receptors are located in the peripheral and central nervous system, in lymphocytes and intestinal enterochromaffine cells2. In presynaptic neurons, activation of these receptors leads to an increase in intracellular Ca2+ (by both influx and mobilization of intracellular stores), and modulates the release of a number of neurotransmitters and neuropeptides2. At the postsynaptic level, activation leads to membrane depolarization2,3. The localization of 5-HT3B subunits is somewhat controversial, some studies show that its expression is restricted to the peripheral nervous system and others show that it is also detected in the brain and in hippocampal neurons1,4-7.5-HT3 receptors have become important targets for which to develop treatments for irritable bowel syndrome (IBS), side effects resulting from chemotherapeutic treatment, schizophrenia and bipolar disorder1,2.

Gene ID: 58963

UniProt: Q9JJ16

Pathways: Synaptic Membrane