Serotonin Receptor 3B antibody (Extracellular, N-Term) (Atto 488)

Catalog No. ABIN7043623

Product Details anti-Serotonin Receptor 3B Antibody (Atto 488)

Target: Serotonin Receptor 3B (HTR3B)

Binding Specificity: AA 213-226, Extracellular, N-Term

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Serotonin Receptor 3B antibody is conjugated to Atto 488

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Characteristics: Anti-5HT3B Receptor (HTR3B) (extracellular) Antibody (ABIN7043622 and ABIN7045285)) is a highly specific antibody directed against an epitope of rat Serotonin receptor 3B. The antibody can be used in western blot and immunohistochemistry applications. It recognizes an extracellular epitope and can potentially detect 5-HT3B in living cells. It has been designed to recognize 5-HT3B from rat and mouse samples. The antibody is unlikely to recognize the receptor from human samples. \nAnti-5HT3B Receptor (HTR3B) (extracellular)-ATTO Fluor-488 Antibody (#ABIN7043623) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-5HT3B Receptor (HTR3B) (extracellular)-ATTO Fluor-488 Antibody is especially suited for experiments requiring simultaneous labeling of different markers.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)HIRQSSAGDFAQIR, corresponding to amino acid residues 213-226 of rat 5-Hydroxytryptamine receptor 3B

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 50 μL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1 % BSA, 0.05 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Serotonin Receptor 3B

Target: Serotonin Receptor 3B (HTR3B)

Alternative Name: 5HT3B Receptor (HTR3B) (HTR3B Products)

Synonyms: HTR3B antibody, 5-HT3B antibody, 5-hydroxytryptamine receptor 3B antibody, 5-hydroxytryptamine (serotonin) receptor 3B antibody, HTR3B antibody, Htr3b antibody

Background: Alternative names: 5HT3B Receptor (HTR3B), 5-Hydroxytryptamine receptor 3B, Serotonin receptor 3B

Gene ID: 58963

NCBI Accession: NM_006028

UniProt: Q9JJ16

Pathways: Synaptic Membrane