S1PR1 antibody (Extracellular, N-Term)

Catalog No. ABIN7885536

This Rabbit Polyclonal antibody specifically detects S1PR1 in WB, IF, IHC, FACS, LCI and IC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for S1PR1 antibody (Extracellular, N-Term) (ABIN7885536)

Target: S1PR1 (Sphingosine-1-Phosphate Receptor 1 (S1PR1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This S1PR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI), Immunochromatography (IC)

Grade: Carrier-free

Product Details anti-S1PR1 Antibody

Binding Specificity: AA 17-30, Extracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to Sphingosine 1-Phosphate Receptor 1

Predicted Reactivity: Mouse,rat - 13,14 amino acid residues identical

Purification: Affinity purified on immobilized antigen.

Immunogen: SDYVNYDIIVRHYN(C), corresponding to amino acid residues 17-30 of human S1PR1

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 5 mg per reaction

ICC: 1:50

IHC: 1:100

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7237267)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for S1PR1

Target: S1PR1 (Sphingosine-1-Phosphate Receptor 1 (S1PR1))

Alternative Name: Sphingosine 1-phosphate receptor 1

Background:

Synonyms: Sphingosine 1-phosphate receptor 1, S1P1, Endothelial differentiation G-protein coupled receptor 1, CD363, CHEDG1

Description: Lysophospholipids constitute a group of important lipid mediators, lysophosphatidic acid (LPA) a glycerolysophospholipid and sphingosine 1-phosphate (S1P), a lysosphingolipid. Sphingosine 1-phosphate is a bioactive lipid derived from metabolism of sphingomyelin1. Sphingosine 1-phosphate is implicated in the regulation of many cellular functions including proliferation, apoptosis, survival, adhesion, differentiation, and migration2. Sphingosine 1-phosphate exerts its activity through five distinct G-protein-coupled receptors, (also named endothelial differentiation gene receptors - EDG), S1PR1 (EDG-1), S1PR2 (EDG-5), S1PR3 (EDG-3), S1PR4 (EDG-6), and S1PR5 (EDG-8)3.Sphingosine 1-phosphate receptor 1 (S1PR1) is ubiquitously expressed. S1PR1 mRNA is detected in the brain, heart, spleen, liver, lung, thymus, kidney, skeletal muscle and lymphoid tissues4. Knockout of the gene encoding S1PR1 is lethal and therefore makes in vivo studies difficult5.

Gene ID: 1901

UniProt: P21453

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2