TACR1 antibody (2nd Extracellular Loop)

Catalog No. ABIN7885566

This Rabbit Polyclonal antibody specifically detects TACR1 in WB, IHC, FACS, LCI and IC. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for TACR1 antibody (2nd Extracellular Loop) (ABIN7885566)

Target: TACR1 (Tachykinin Receptor 1 (TACR1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TACR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI), Immunochromatography (IC)

Grade: Carrier-free

Product Details anti-TACR1 Antibody

Binding Specificity: 2nd Extracellular Loop, AA 180-194

Purpose: A Rabbit Polyclonal Antibody to Neurokinin 1 Receptor

Predicted Reactivity: Mouse - identical, human - 13,15 amino acid residues identical

Purification: Affinity purified on immobilized antigen.

Immunogen: CMIEWPEHPNRTYEK, corresponding to amino acid residues 180-194 of rat NK1 receptor 

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 2.5 mg per reaction

ICC: 1:50

IHC: 1:100

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7237370)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for TACR1

Target: TACR1 (Tachykinin Receptor 1 (TACR1))

Alternative Name: NK1 receptor

Background:

Synonyms: NK1 receptor, Tachykinin receptor 1, TACR1, Substance-P receptor, SPR

Description: Substance P (SP), Neurokinin A (NKA) and Neurokinin B (NKB) are all peptides belonging to the Tachykinin protein family. These three peptides, which demonstrate quite a heterogeneity in their distribution, exert their effect via three receptors: Neurokinin 1-3 receptors, members of the G-protein coupled receptor superfamily. However, Neurokinin 1 (NK1) receptor preferentially binds Substance P, Neurokinin 2 (NK2) receptor to NKA, and Neurokinin 3 (NK3) receptor to NKB1.Neurokinin receptors are distinguished by their seven transmembrane domains, an extracellular N-terminus and a cytosolic C-terminal. An unusual property of these receptors is the presence of introns as part of their structural organization1-3. Tachykinin receptors undergo alternative splicing. For example, NK1 receptor is detected with different C-terminal lengths. The longer receptor isoform is found in the brain whereas the truncated form is mostly detected in the periphery1,4.Due to the broad expression profile of tachykinin peptides, their respective receptors are also expressed in a similar fashion. NK1 receptor is widely expressed in neurons, endothelial cells, muscle and immune system cells. NK2 receptor is broadly expressed in the periphery and its expression in the brain is quite restricted. NK3 receptor on the other hand is largely expressed in the central nervous system and is also detected in the uterus, skeletal muscle, lung and liver1.Neurokinin receptors have been found in many pathophysiological indications and have therefore become targets for the development of pharmacological compounds. Such indications include cancer, psychological disorders, migraine and various inflammations, just to name a few5-8.

Gene ID: 24807

UniProt: P14600

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour, Smooth Muscle Cell Migration