Tetraspanin 7 antibody (Extracellular, Loop 2)

Catalog No. ABIN7885573

The Rabbit Polyclonal anti-Tetraspanin 7 antibody is suitable to detect Tetraspanin 7 in samples from Human, Mouse and Rat. It has been validated for WB, FACS, IHC and LCI.

Quick Overview for Tetraspanin 7 antibody (Extracellular, Loop 2) (ABIN7885573)

Target: Tetraspanin 7 (TSPAN7)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Tetraspanin 7 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Live Cell Imaging (LCI)

Grade: Carrier-free

Product Details anti-Tetraspanin 7 Antibody

Binding Specificity: AA 155-169, Extracellular, Loop 2

Purpose: A Rabbit Polyclonal Antibody to Tetraspanin 7 (extracellular)

Predicted Reactivity: Mouse - 15 out of 16 amino acid residues identical, rat - 14 out of 16 amino acid residues identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)DWENTDWFKETKNQS, corresponding to amino acid residues 155 - 169 of human Tetraspanin-3

Isotype: IgG

Application Details

Application Notes:

WB: 1:400-1:500

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:300

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7237381)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Tetraspanin 7

Target: Tetraspanin 7 (TSPAN7)

Alternative Name: TSPAN7

Background:

Synonyms: TSPAN7, Transmembrane 4 Superfamily Member 2, TM4SF2, T-Cell Acute Lymphoblastic Leukemia-Associated Antigen 1, TALLA-1, Cell Surface Glycoprotein A15, A15, CD231.

Description: Tetraspanins (Tspans) are a family of four-span transmembrane proteins, known as plasma membrane "master organizers." They form Tspan-enriched microdomains (TEMs or TERMs) through lateral association with one another and other membrane proteins.1Tetraspanins are characterized by four transmembrane domains, two extracellular loops: one small extracellular loop (SEL or EC1) and one larger extracellular loop (LEL or EC2), a short intracellular loop, and two short intracellular tails.1Teatraspanin 7 (TSPAN7) is highly expressed in the brain and was first described as a candidate gene for X-linked intellectual disability, probably through the modulation of surface expression of AMPA and dopamine receptors.2Tetraspanin 7 also has a key role in controlling pancreatic β-cell Ca2+ handling and defining the set-point of insulin secretion through modulation of L-type Cav1.2 and Cav1.3 channels. Furthermore, it has been identified as a target for autoantibodies in Type I diabetes. 3In addition, Tetraspanin 7 is involved in the regulation of HIV virus entry into dendritic cells and several types of cancer progression. 2

Gene ID: 7102

UniProt: P41732