TMEM38A antibody (C-Term, Intracellular)

Catalog No. ABIN7885621

The Rabbit Polyclonal anti-TMEM38A antibody is suitable to detect TMEM38A in samples from Rat and Mouse. It has been validated for WB, IHC and IC.

Quick Overview for TMEM38A antibody (C-Term, Intracellular) (ABIN7885621)

Target: TMEM38A (Transmembrane Protein 38A (TMEM38A))

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TMEM38A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunochromatography (IC)

Grade: Carrier-free

Product Details anti-TMEM38A Antibody

Binding Specificity: AA 259-274, C-Term, Intracellular

Purpose: A Rabbit Polyclonal Antibody to TRIC-A Channel

Predicted Reactivity: Mouse - identical, human - will not recognize human TRIC-A

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)DNHGAPHGMGLGTQHS, corresponding to amino acid residues 259-274 of rat TRIC-A

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: 1:200

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7237521)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for TMEM38A

Target: TMEM38A (Transmembrane Protein 38A (TMEM38A))

Alternative Name: Trimeric intracellular cation channel type A

Background:

Synonyms: Trimeric intracellular cation channel type A, 27 kDa sarcoplasmic reticulum protein, Transmembrane protein 38A

Description: Intracellular Ca2+ levels are important in proper cellular functions and have prominent roles in various cell signaling pathways and are crucial for muscle contractions. Indeed, an important step leading to muscle contraction is the massive release of Ca2+ ions from the endoplasmic/sarcoplasmic reticulum (ER/SR) to the cytosol. A battery of results suggest that specific K+ channels are important to counteract the Ca2+ outflow in order to neutralize the negative potential created by the movement of Ca2+ ions. It is believed that TRIC channels are responsible for neutralizing this negative potential1,2.Trimeric intracellular cation-specific (TRIC) channels are critical for proper management of intracellular stores. TRIC-A and TRIC-B both belong to this family and are both permeable to monovalent ions with a preference for K+ 3. Both channels are localized to the ER/SR membrane. Each TRIC subunit contains three transmembrane domains, a cytoplasmic C-terminus and a luminal N-terminus. Functional entities are formed by homotrimerizarion4. The activity of TRIC-A is regulated by voltage whereas that of TRIC-B can be regulated by different mechanisms3.Knockout studies of these channels have shown that TRIC-A knockout mice are viable while those of TRIC-B die at the neonatal stage5.TRIC-A is mostly expressed in excitable tissues like the brain and muscle while TRIC-B is ubiquitously expressed.

Gene ID: 306327

UniProt: A6ZIQ8