RAF1 antibody (pSer259) (Biotin)

Catalog No. ABIN745390

This Rabbit Polyclonal antibody specifically detects RAF1 in WB, ELISA and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for RAF1 antibody (pSer259) (Biotin) (ABIN745390)

Target: RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAF1 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-RAF1 Antibody (Biotin)

Binding Specificity: pSer259

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Sheep,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human c-Raf around the phosphorylation site of Ser259

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for RAF1

Target: RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))

Alternative Name: c-Raf

Background:

Synonyms: RAF1phospho S259, Raf1Ser259, RAF1, murine leukemia viral v-raf-1 oncogene homolog 1 3611-MSV, v-raf-1 murine leukemia viral oncogene homolog 1, cRaf, C-RAF, proto-oncogene c-RAF, 6430402F14Rik, AA990557, BB129353, c-Raf, Craf1, D830050J10Rik, MGC102375, Raf-1, Raf 1, v-Raf, c Raf, Craf 1 transforming gene, Craf1 transforming gene, EC 2.7.11.1, Murine sarcoma 3611 oncogene 1, RAF, Raf proto oncogene serine/threonine protein kinase, vraf1 murine leukemia viral oncogene homolog 1, c-Raf.

Background: The Raf family of serine/threonine specific kinases is comprised of three members (aRaf, bRaf, and cRaf) that play a critical role in regulating cell growth and differentiation, and couple growth factor receptor stimulation to nuclear transcription factors via the Ras/mitogen activated protein kinase (MAPK) pathway. cRaf kinase (also known as Raf1) is a small GTPase like kinase of 73 kDa, and is a signal transducer of multiple extracellular stimuli that is regulated by several pathways, and that once activated, phosphorylates MEK which in turn phosphorylates ERK. Raf1 is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It is part of the Ras dependent signaling pathway from receptors to the nucleus.

Gene ID: 5894

Pathways: MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling, BCR Signaling