NR1D1 antibody (pSer55, pSer59)

Catalog No. ABIN745553

The Rabbit Polyclonal anti-NR1D1 antibody is suitable to detect NR1D1 in samples from Human. It has been validated for WB, ELISA, IHC (p), IF (cc), IF (p) and IHC (fro).

Quick Overview for NR1D1 antibody (pSer55, pSer59) (ABIN745553)

Target: NR1D1 (Nuclear Receptor Subfamily 1, Group D, Member 1 (NR1D1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NR1D1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-NR1D1 Antibody

Binding Specificity: pSer55, pSer59

Specificity: These phosphorylation sites are homologous among the listed cross-reactive species.

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Cow,Sheep

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human REV-ERB alpha around the phosphorylation site of Ser55/59

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for NR1D1

Target: NR1D1 (Nuclear Receptor Subfamily 1, Group D, Member 1 (NR1D1))

Alternative Name: NR1D1 +

Background:

Synonyms: EAR1, hRev, THRA1, THRAL, ear-1, Nuclear receptor subfamily 1 group D member 1, Rev-erbA-alpha, V-erbA-related protein 1, NR1D1

Background: This gene encodes a transcription factor that is a member of the nuclear receptor subfamily 1. The encoded protein is a ligand-sensitive transcription factor that negatively regulates the expression of core clock proteins. In particular this protein represses the circadian clock transcription factor aryl hydrocarbon receptor nuclear translocator-like protein 1 (ARNTL). This protein may also be involved in regulating genes that function in metabolic, inflammatory and cardiovascular processes. [provided by RefSeq, Jan 2013]

Gene ID: 9572

UniProt: P20393

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Lipid Metabolism by PPARalpha