NR1D1 antibody (pSer55, pSer59)

Catalog No. ABIN745553

Product Details anti-NR1D1 Antibody

Target: NR1D1 (Nuclear Receptor Subfamily 1, Group D, Member 1 (NR1D1))

Binding Specificity: pSer55, pSer59

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NR1D1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: These phosphorylation sites are homologous among the listed cross-reactive species.

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Cow,Sheep

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human REV-ERB alpha around the phosphorylation site of Ser55/59

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for NR1D1

Target: NR1D1 (Nuclear Receptor Subfamily 1, Group D, Member 1 (NR1D1))

Alternative Name: NR1D1 + (NR1D1 Products)

Synonyms: MGC82865 antibody, MGC82881 antibody, Eip75B antibody, EAR1 antibody, THRA1 antibody, THRAL antibody, ear-1 antibody, hRev antibody, A530070C09Rik antibody, R75201 antibody, REV-ERBAALPHA antibody, nuclear receptor subfamily 1 group D member 1 L homeolog antibody, nuclear receptor subfamily 1, group d, member 1 antibody, nuclear receptor subfamily 1 group D member 1 antibody, nuclear receptor subfamily 1, group D, member 1 antibody, nr1d1.L antibody, nr1d1 antibody, NR1D1 antibody, Nr1d1 antibody

Background:

Synonyms: EAR1, hRev, THRA1, THRAL, ear-1, Nuclear receptor subfamily 1 group D member 1, Rev-erbA-alpha, V-erbA-related protein 1, NR1D1

Background: This gene encodes a transcription factor that is a member of the nuclear receptor subfamily 1. The encoded protein is a ligand-sensitive transcription factor that negatively regulates the expression of core clock proteins. In particular this protein represses the circadian clock transcription factor aryl hydrocarbon receptor nuclear translocator-like protein 1 (ARNTL). This protein may also be involved in regulating genes that function in metabolic, inflammatory and cardiovascular processes. [provided by RefSeq, Jan 2013]

Gene ID: 9572

UniProt: P20393

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Lipid Metabolism by PPARalpha