SIRT1 antibody (pSer47)

Catalog No. ABIN745658

The Rabbit Polyclonal anti-SIRT1 antibody has been validated for WB, ELISA, FACS, IF (cc), IF (p), IHC (p) and IHC (fro). It is suitable to detect SIRT1 in samples from Human, Mouse, Rat and Dog. There are 4+ publications available.

Quick Overview for SIRT1 antibody (pSer47) (ABIN745658)

Target: SIRT1 (Sirtuin 1 (SIRT1))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIRT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-SIRT1 Antibody

Binding Specificity: pSer47

Cross-Reactivity: Dog, Human, Mouse, Rat

Predicted Reactivity: Pig,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human SirT1 around the phosphorylation site of Ser47

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-SIRT1 antibody (ABIN745658)

Liu, Hou, Yuan, Hang, Zhao, Sun, Zhao, Zhao, Dong, Wang, Shi, Liu, Zhou, Dong, Liu, Zhan, Li, Li: "Fenofibrate inhibits atrial metabolic remodelling in atrial fibrillation through PPAR-α/sirtuin 1/PGC-1α pathway." in: British journal of pharmacology, Vol. 173, Issue 6, pp. 1095-109, (2016) (PubMed).

Suzuki, Bandoski, Bartlett: "Fluoride induces oxidative damage and SIRT1/autophagy through ROS-mediated JNK signaling." in: Free radical biology & medicine, Vol. 89, pp. 369-378, (2015) (PubMed).

Suzuki, Bartlett: "Sirtuin1 and autophagy protect cells from fluoride-induced cell stress." in: Biochimica et biophysica acta, Vol. 1842, Issue 2, pp. 245-55, (2014) (PubMed).

Target Details for SIRT1

Target: SIRT1 (Sirtuin 1 (SIRT1))

Alternative Name: SIRT1

Background:

Synonyms: SIR2L1, NAD-dependent protein deacetylase sirtuin-1, hSIRT1, Regulatory protein SIR2 homolog 1, SIR2-like protein 1, hSIR2, SIRT1

Background: This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined, however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants.

Gene ID: 23411

UniProt: Q96EB6

Pathways: MAPK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling