PTK2B antibody (pTyr881) (HRP)

Catalog No. ABIN745787

The Rabbit Polyclonal anti-PTK2B antibody is suitable to detect PTK2B in samples from Mouse and Rat. It has been validated for WB, ELISA, IHC (p) and IHC (fro).

Quick Overview for PTK2B antibody (pTyr881) (HRP) (ABIN745787)

Target: PTK2B (PTK2B Protein tyrosine Kinase 2 beta (PTK2B))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PTK2B antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-PTK2B Antibody (HRP)

Binding Specificity: pTyr881

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human Pyk2 around the phosphorylation site of Tyr881

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PTK2B

Target: PTK2B (PTK2B Protein tyrosine Kinase 2 beta (PTK2B))

Alternative Name: Pyk2

Background:

Synonyms: CADTK, CAK beta , CAKB antibody, CAKbeta, Calcium dependent tyrosine kinase, Calcium-dependent tyrosine kinase, Cell adhesion kinase beta, E430023O05Rik, EC 2.7.10.2, FADK 2, FADK2, FAK 2, FAK1, FAK2, Focal Adhesion Kinase 2, MGC124628, PKB, pp125FAK, Proline Rich Tyrosine Kinase 2, Proline-rich tyrosine kinase 2, Protein kinase B, Protein Tyrosine Kinase 2 Beta, PTK 2B, PTK antibody PTK2B, PTK2B protein tyrosine kinase 2 beta, PYK 2, PYK2, RAFTK, Related adhesion focal tyrosine kinase.

Background: PYK2 is involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. PKY2 may represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage gated potassium channel protein Kv1.2. PYK2 activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity.

Gene ID: 2185

Pathways: EGFR Signaling Pathway, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Cell-Cell Junction Organization, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGF Signaling