NCOA3 antibody (pThr24) (HRP)

Catalog No. ABIN745892

The Rabbit Polyclonal anti-NCOA3 antibody has been validated for ELISA, IHC (p) and IHC (fro). It is suitable to detect NCOA3 in samples from Human.

Quick Overview for NCOA3 antibody (pThr24) (HRP) (ABIN745892)

Target: NCOA3 (Nuclear Receptor Coactivator 3 (NCOA3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCOA3 antibody is conjugated to HRP

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-NCOA3 Antibody (HRP)

Binding Specificity: pThr24

Cross-Reactivity: Human

Predicted Reactivity: Pig,Horse,Rabbit,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human SRC-3 around the phosphorylation site of Thr24

Isotype: IgG

Application Details

Application Notes: IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for NCOA3

Target: NCOA3 (Nuclear Receptor Coactivator 3 (NCOA3))

Alternative Name: Src-3

Background:

Synonyms: ACTR, AIB 1, AIB-1, AIB1, Amplied in breast cancer 1, Amplied in breast cancer 1 protein, bHLHe42, CAGH 16, CAGH16, CBP interacting protein, CBP-interacting protein, Class E basic helix-loop-helix protein 42, CTG 26, CTG26, KAT13B / AIB1, MGC141848, NCOA 3, NCOA 3, NCoA-3, Ncoa3, NCOA3_HUMAN, Nuclear receptor coactivator 3, pCIP, RAC 3, RAC-3, RAC3, Receptor associated coactivator 3, Receptor-associated coactivator 3, SRC 3, SRC-3, SRC3, Steroid receptor coactivator protein 3, Thyroid hormone receptor activator molecule 1, TNRC14, TNRC16, TRAM 1, TRAM-1, TRAM1.

Background: KAT13B/AIB1 interacts in a ligand-dependent fashion with the estrogen receptor to enhance estrogen-dependent transcriptional gene activation. The protein encoded by this gene is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. The encoded protein has histone acetyltransferase activity and recruits KAT3A / KAT3B as part of a multisubunit coactivation complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. Two transcript variants encoding different isoforms have been found for this gene. In addition, a polymorphic repeat region is found in the C-terminus of the encoded protein.

Gene ID: 8202

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha