CRTC2 antibody (pSer171)

Catalog No. ABIN745988

The Rabbit Polyclonal anti-CRTC2 antibody has been validated for WB, ELISA and FACS. It is suitable to detect CRTC2 in samples from Human and Mouse. There are 4+ publications available.

Quick Overview for CRTC2 antibody (pSer171) (ABIN745988)

Target: CRTC2 (CREB Regulated Transcription Coactivator 2 (CRTC2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CRTC2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-CRTC2 Antibody

Binding Specificity: pSer171

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Dog,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human CREB regulated transcription coactivator 2 around the phosphorylation site of Ser171

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-CRTC2 antibody (ABIN745988)

Slocum, Skoko, Wakabayashi, Aja, Yamamoto, Kensler, Chartoumpekis: "Keap1/Nrf2 pathway activation leads to a repressed hepatic gluconeogenic and lipogenic program in mice on a high-fat diet." in: Archives of biochemistry and biophysics, Vol. 591, pp. 57-65, (2016) (PubMed).

Seok, Fu, Choi, Li, Zhu, Kumar, Sun, Yoon, Kang, Zhong, Ma, Kemper, Kemper: "Transcriptional regulation of autophagy by an FXR-CREB axis." in: Nature, Vol. 516, Issue 7529, pp. 108-11, (2015) (PubMed).

Tian, Zhao, Sun, Zhi, Cheng, Zhou, Hu: "CRTC2 enhances HBV transcription and replication by inducing PGC1? expression." in: Virology journal, Vol. 11, Issue 1, pp. 30, (2014) (PubMed).

Cicerchi, Li, Kratzer, Garcia, Roncal-Jimenez, Tanabe, Hunter, Rivard, Sautin, Gaucher, Johnson, Lanaspa: "Uric acid-dependent inhibition of AMP kinase induces hepatic glucose production in diabetes and starvation: evolutionary implications of the uricase loss in hominids." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 28, Issue 8, pp. 3339-50, (2014) (PubMed).

Target Details for CRTC2

Target: CRTC2 (CREB Regulated Transcription Coactivator 2 (CRTC2))

Alternative Name: TORC2

Background:

Synonyms: TORC2, TORC-2, CREB-regulated transcription coactivator 2, Transducer of regulated cAMP response element-binding protein 2, Transducer of CREB protein 2, CRTC2

Background: Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. Regulates the expression of specific genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR).

Gene ID: 200186

UniProt: Q53ET0

Pathways: AMPK Signaling, Carbohydrate Homeostasis