SMAD3 antibody (SMAD, Mothers Against DPP Homolog 3) (pSer423)

Details for Product anti-SMAD3 Antibody No. ABIN746138, Supplier: Log in to see
Antigen
  • madh3
  • madh3a
  • smad3
  • wu:fa99e03
  • HSPC193
  • HsT17436
  • JV15-2
  • LDS1C
  • LDS3
  • MADH3
  • XSmad3
  • XenMLP
  • Xmad3
  • madh3-A
  • Madh3
  • AU022421
  • Smad 3
  • mad3
  • SMAD family member 3
  • SMAD family member 3a
  • SMAD family member 3 L homeolog
  • SMAD3
  • smad3a
  • smad3.L
  • Smad3
Alternatives
anti-Human SMAD3 antibody for Western Blotting
Epitope
pSer423, pSer425
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60
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49
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36
22
22
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12
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11
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2
2
2
2
1
1
1
1
1
1
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1
1
1
1
1
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1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
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333
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57
48
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39
32
23
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18
4
3
3
2
2
1
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1
Host
Rabbit
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64
15
Clonality
Polyclonal
Conjugate
This SMAD3 antibody is un-conjugated
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22
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12
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7
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3
3
3
1
1
1
1
1
1
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Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
487
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112
66
57
36
31
19
18
9
7
2
2
1
1
Options
Supplier
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Supplier Product No.
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Immunogen KLH conjugated synthetic phosphopeptide derived from human Smad3 around the phosphorylation site of Ser423/425 [CS(p-S)V(p-S)]
Isotype IgG
Specificity These phosphorylation sites are homologous across the species listed.
Purification Purified by Protein A.
Plasmids, Primers & others Plasmids, Primers & others SMAD3 products on genomics-online (e.g. as negative or positive controls)
Alternative Name Smad3 (SMAD3 Antibody Abstract)
Background

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

Subcellular location: Cytoplasm, Nucleus

Synonyms: LDS3, LDS1C, MADH3, JV15-2, HSPC193, HsT17436, Mothers against decapentaplegic homolog 3, MAD homolog 3, Mad3, Mothers against DPP homolog 3, hMAD-3, SMAD family member 3, SMAD 3, Smad3, hSMAD3

Gene ID 4088
UniProt P84022
Pathways Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy
Application Notes WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Restrictions For Research Use only
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months
Supplier Images
SDS-PAGE (SDS) image for anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (pSer423), (pSer425) antibody (ABIN746138) Lane 1: mouse embryo lysates Lane 2: mouse brain lysates probed with Anti Phospho-Sma...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (pSer423), (pSer425) antibody (ABIN746138) Formalin-fixed and paraffin embedded mouse uterus labeled with Anti-Smad3 (Ser423 + S...
Product cited in: Bai, Chu, Wang, Xiang, Yang: "BAMBI promotes porcine granulosa cell steroidogenesis involving TGF-β signaling." in: Theriogenology, Vol. 100, pp. 24-31, 2017 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Pig (Porcine)).

Shang, Liu, Liu, Zhang, Mu, Zhu, Liang, Zhai, Ding, Li, Wen: "Acetyl-11-Keto-β-Boswellic Acid Attenuates Prooxidant and Profibrotic Mechanisms Involving Transforming Growth Factor-β1, and Improves Vascular Remodeling in Spontaneously Hypertensive Rats." in: Scientific reports, Vol. 6, pp. 39809, 2016 (PubMed). Method employed by authors: Western Blotting (WB), Western Blotting (WB), Western Blotting (WB) (Sample species: Rat (Rattus)).

Ghiabi, Jiang, Pasquier, Maleki, Abu-Kaoud, Halabi, Guerrouahen, Rafii, Rafii: "Breast cancer cells promote a notch-dependent mesenchymal phenotype in endothelial cells participating to a pro-tumoral niche." in: Journal of translational medicine, Vol. 13, Issue 1, pp. 27, 2015 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

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