RFP antibody (Alexa Fluor 750)

Catalog No. ABIN7937948

The Alpaca Monoclonal anti-RFP antibody is suitable to detect RFP in samples from Discosoma. It has been validated for IF, ICC and FACS.

Quick Overview for RFP antibody (Alexa Fluor 750) (ABIN7937948)

Target: RFP (Red Fluorescent Protein (RFP))

Reactivity: Discosoma

Host: Alpaca

Clonality: Monoclonal

Conjugate: This RFP antibody is conjugated to Alexa Fluor 750

Application: Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 2B12-2A1

Product Details anti-RFP Antibody (Alexa Fluor 750)

Purpose: Camelid sdAb anti-RFP Alexa Fluor™ 750, Clone(s)2B12 / 2A1

Immunogen: mRFP and derivatives like mCherry, mScarlet-i, tdTomato, dsRed, mOrange

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

A blend of 2 different sdAbs that bind at different epitopes of the same target protein, two fluorophore molecules are site-specifically coupled to each individual sdAb molecule. The reagent therefore simultaneously targets up to four fluorophores to the protein of interest, which ensures extra-bright signals. Owing to the small size of sdAb, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, this product can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render this products excellent tools for all microscopy techniques.

Restrictions: For Research Use only

Handling

Concentration: 5 μM

Storage: -80 °C

Storage Comment: -80°C

Target Details for RFP

Target: RFP (Red Fluorescent Protein (RFP))

Alternative Name: mRFP