BAZ2A antibody (N-Term)

Catalog No. ABIN7938055

The Rabbit Polyclonal anti-BAZ2A antibody (ABIN7938055) specifically detects BAZ2A in WB and IF. The antibody is reactive with Human samples.

Quick Overview for BAZ2A antibody (N-Term) (ABIN7938055)

Target: BAZ2A (Bromodomain Adjacent To Zinc Finger Domain, 2A (BAZ2A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BAZ2A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-BAZ2A Antibody

Binding Specificity: N-Term

Purpose: BAZ2A antibody (pAb)

Purification: Affinity Purified

Immunogen: This antibody was raised against a peptide within the N-terminal region of human BAZ2A.

Isotype: IgG

Application Details

Application Notes: WB*: 1:500 - 1:1,000 dilution The addition of 0.1 % Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required. *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for BAZ2A

Target: BAZ2A (Bromodomain Adjacent To Zinc Finger Domain, 2A (BAZ2A))

Alternative Name: BAZ2A

Background:

Background_Plain: BAZ2A (Bromodomain Adjacent To Zinc Finger Domain, 2A) is an essential component of the NoRC (nucleolar remodeling complex) complex, a complex that mediates silencing of a fraction of rDNA by recruiting histone-modifying enzymes and DNA methyltransferases, leading to heterochromatin formation and transcriptional silencing. In the complex, it plays a central role by being recruited to rDNA and by targeting chromatin modifying enzymes such as HDAC1, leading to repress RNA polymerase I transcription. Recruited to rDNA via its interaction with TTF1 and its ability to recognize and bind histone H4 acetylated on 'Lys-16' (H4K16ac), leading to deacetylation of H4K5ac, H4K8ac, H4K12ac but not H4K16ac. Specifically binds pRNAs, 150-250 nucleotide RNAs that are complementary in sequence to the rDNA promoter, pRNA-binding is required for heterochromatin formation and rDNA silencing (By similarity).

Aliases: TIP5, WALp3

Molecular Weight: 270 kDa

NCBI Accession: NP_038477