BRD4 antibody (C-Term)

Catalog No. ABIN7938067

The Rabbit Polyclonal anti-BRD4 antibody is suitable to detect BRD4 in samples from Human. It has been validated for WB.

Quick Overview for BRD4 antibody (C-Term) (ABIN7938067)

Target: BRD4 (Bromodomain Containing 4 (BRD4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRD4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-BRD4 Antibody

Binding Specificity: C-Term

Purpose: BRD4 antibody (pAb)

Purification: Protein A Chromatography

Immunogen: This BRD4 antibody was raised against a peptide within the C-terminal region of human BRD4.

Isotype: IgG

Application Details

Application Notes: *WB: 0.5 - 2.0 μg/mL dilution *For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.05 % Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.

Restrictions: For Research Use only

Handling

Buffer: Purified rabbit IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for BRD4

Target: BRD4 (Bromodomain Containing 4 (BRD4))

Alternative Name: BRD4

Background:

Background_Plain: BRD4 (Bromodomain-containing protein 4, aka HUNK1) is a chromatin-associated protein with two bromo domains that binds acetylated histone H3 and H4. BRD4 is homologous to Xenopus MCAP, and like MCAP, BRD4 associates with chromosomes during mitosis, predominantly in the non-centromeric regions. A chromosomal aberration involving BRD4 (producing a BRD4-NUT fusion protein) is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. BRD4 is involved in the expression of extracellular matrix gene expression, and its misregulation may be involved in breast cancer progression.

Aliases: CAP, MCAP, HUNK1, HUNKI

Molecular Weight: 180 kDa

NCBI Accession: NP_490597

Pathways: Chromatin Binding, SARS-CoV-2 Protein Interactome