BRD7 antibody (C-Term)

Catalog No. ABIN7938068

The Rabbit Polyclonal anti-BRD7 antibody is suitable to detect BRD7 in samples from Human. It has been validated for WB.

Quick Overview for BRD7 antibody (C-Term) (ABIN7938068)

Target: BRD7 (Bromodomain Containing 7 (BRD7))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRD7 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-BRD7 Antibody

Binding Specificity: C-Term

Purpose: BRD7 antibody (pAb)

Purification: Affinity Purified

Immunogen: This BRD7 antibody was raised against a peptide within the C-terminal region of human BRD7.

Isotype: IgG

Application Details

Application Notes: WB*: 1:500 - 1:2,000 dilution For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.05 % Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required. *Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in 70 mM Tris, 105 mM NaCl, 0.07 mM EDTA, 31 mM glycine, 0.035 % sodium azide, 30 % glycerol, 0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for BRD7

Target: BRD7 (Bromodomain Containing 7 (BRD7))

Alternative Name: BRD7

Background:

Background_Plain: BRD7 (Bromodomain-containing protein 7, aka CELTIX1) is a chromatin-associated protein component of both functional p53 and BRCA1 (breast cancer 1, early onset) pathways. BRD7 inhibit4ed cancer cell growth and metastasis and promotes apoptosis in vitro and in vivo via downregulating AKT pathway. BRD7 may also regulate signaling pathways like ras-raf-MEK-ERK and RB/E2F. Previous studies have also demonstrated that BRD7 was downregulated in huma breast cancer, and the downregulation is associated with tumor progression.

Aliases: Bromodomain containing 7, CELTIX1, BP75, SMARCI1

Molecular Weight: 75 kDa

NCBI Accession: NM_013263