EZH2 antibody (AA 1-370)

Catalog No. ABIN7938125

The Rabbit Polyclonal anti-EZH2 antibody is suitable to detect EZH2 in samples from Human and Mouse. It has been validated for IP, ChIP, ChIP-seq and CUT&RUN.

Quick Overview for EZH2 antibody (AA 1-370) (ABIN7938125)

Target: EZH2 (Enhancer of Zeste Homolog 2 (EZH2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EZH2 antibody is un-conjugated

Application: Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Release Using Nuclease (CUT&RUN)

Product Details anti-EZH2 Antibody

Binding Specificity: AA 1-370

Purpose: EZH2 antibody (pAb)

Purification: Unpurified

Immunogen: This EZH2 antibody was raised against a recombinant fusion protein corresponding to amino acids 1-370 of mouse EZH2.

Application Details

Application Notes: ChIP-Seq: 5 µL each CUT&RUN: 1 µL per 50 µL reaction This antibody has not been validated in WB, but a WB-validated antibody is available for this target: 39933. For EZH2, we also offer AbFlex EZH2 Recombinant Antibody (rAb). For details, see Catalog No. 91159.

Restrictions: For Research Use only

Handling

Buffer: Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for EZH2

Target: EZH2 (Enhancer of Zeste Homolog 2 (EZH2))

Alternative Name: EZH2

Background:

Background_Plain: EZH2 - (Enhancer of Zeste homolog 2) is a human homolog of the Drosophila Polycomb-group protein Enhancer of Zeste protein. It contains a SET domain that catalyzes the methylation of histone H3 at lysine 27 (Histone H3 Lys27 monomethylated, dimethylated and trimethylated). Polycomb-group proteins repress gene expression by binding to chromatin and locally altering chromatin structure. EZH2, BMI-1 and Suz12 are present in the PRC2 and PRC3 protein complexes that function as mediators of epigenetic transcriptional silencing. EZH2 associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. EZH2 may play a role in the hematopoietic and central nervous systems. Deregulation of EZH2 is linked to a variety of cancers.

Aliases: WVS, ENX1, EZH1, KMT6, WVS2, ENX-1, EZH2b, KMT6A

Molecular Weight: 96 kDa

NCBI Accession: NP_031997

Pathways: Retinoic Acid Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation