EZH2 antibody (AA 1-370)

Catalog No. ABIN7938127

The Rabbit Polyclonal anti-EZH2 antibody (ABIN7938127) specifically detects EZH2 in WB. The antibody is reactive with Human and Mouse samples.

Quick Overview for EZH2 antibody (AA 1-370) (ABIN7938127)

Target: EZH2 (Enhancer of Zeste Homolog 2 (EZH2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EZH2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EZH2 Antibody

Binding Specificity: AA 1-370

Purpose: EZH2 antibody (pAb)

Purification: Affinity Purified

Immunogen: This EZH2 antibody was raised against a recombinant fusion protein corresponding to amino acids 1-370 of mouse EZH2.

Isotype: IgG

Application Details

Application Notes: WB: 1:250-1:1000 dilution For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.05 % Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in 70 mM Tris, 105 mM NaCl, 0.07 mM EDTA, 31 mM glycine, 0.035 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for EZH2

Target: EZH2 (Enhancer of Zeste Homolog 2 (EZH2))

Alternative Name: EZH2

Background:

Background_Plain: EZH2 - (Enhancer of Zeste homolog 2) is a human homolog of the Drosophila Polycomb-group protein Enhancer of Zeste protein. It contains a SET domain that catalyzes the methylation of histone H3 at lysine 27 (Histone H3 Lys27 monomethylated, dimethylated and trimethylated). Polycomb-group proteins repress gene expression by binding to chromatin and locally altering chromatin structure. EZH2, BMI-1 and Suz12 are present in the PRC2 and PRC3 protein complexes that function as mediators of epigenetic transcriptional silencing. EZH2 associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. EZH2 may play a role in the hematopoietic and central nervous systems. Deregulation of EZH2 is linked to a variety of cancers.

Aliases: WVS, ENX1, EZH1, KMT6, WVS2, ENX-1, EZH2b, KMT6A

Molecular Weight: 90 kDa

Pathways: Retinoic Acid Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation