HIRA antibody

Catalog No. ABIN7938156

The Mouse Monoclonal anti-HIRA antibody (Clone WC119-2H11) (ABIN7938156) specifically detects HIRA in WB, IF and ICC. The antibody is reactive with Human and Mouse samples.

Quick Overview for HIRA antibody (ABIN7938156)

Target: HIRA (HIR Histone Cell Cycle Regulation Defective Homolog A (HIRA))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This HIRA antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: WC119-2H11

Product Details anti-HIRA Antibody

Purpose: HIRA antibody (mAb) (Clone WC119.2H11)

Purification: Protein G Chromatography

Immunogen: This HIRA antibody was raised against a GST-fusion protein containing residues 421-729 of human HIRA.

Isotype: IgG

Application Details

Application Notes: WB: 1 - 2 μg/mL dilution For optimal results, we recommend the addition of 0.05 % Tween-20 to all blocking solutions to reduce background in Western blot.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for HIRA

Target: HIRA (HIR Histone Cell Cycle Regulation Defective Homolog A (HIRA))

Alternative Name: HIRA

Background:

Background_Plain: HIRA (DGCR1, TUP1) is a histone chaperone that preferentially places the variant Histone H3.3 in nucleosomes, replacing canonical Histone H3 during the cell cycle. HIRA is required for the periodic repression of histone gene transcription during the cell cycle and when complexed with ASF1a is involved in the formation of the senescence-associated heterochromatin foci.

Aliases: TUP1, DGCR1, TUPLE1

Molecular Weight: 120 kDa

NCBI Accession: NP_003316

Pathways: Chromatin Binding