Histone H2A antibody

Catalog No. ABIN7938160

This Rabbit Polyclonal antibody specifically detects Histone H2A in WB, ChIP and IP. It exhibits reactivity toward Saccharomyces cerevisiae and Schizosaccharomyces pombe.

Quick Overview for Histone H2A antibody (ABIN7938160)

Target: Histone H2A

Reactivity: Saccharomyces cerevisiae, Schizosaccharomyces pombe

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H2A antibody is un-conjugated

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP)

Product Details anti-Histone H2A Antibody

Purpose: Histone H2A antibody (pAb)

Purification: Unpurified

Immunogen: This Histone H2A antibody was raised against recombinant yeast histone H2A.

Application Details

Application Notes: WB: 1:2,000 - 1:5,000 dilution

Restrictions: For Research Use only

Handling

Buffer: Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for Histone H2A

Target: Histone H2A

Background: Histone H2A is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression.

Molecular Weight: 14 kDa

NCBI Accession: NP_010511