Histone H2A antibody (H2AK119ub)

Catalog No. ABIN7938165

The Rabbit Polyclonal anti-Histone H2A antibody is suitable to detect Histone H2A in samples from Human. It has been validated for WB and DB.

Quick Overview for Histone H2A antibody (H2AK119ub) (ABIN7938165)

Target: Histone H2A

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H2A antibody is un-conjugated

Application: Western Blotting (WB), Dot Blot (DB)

Product Details anti-Histone H2A Antibody

Binding Specificity: H2AK119ub

Purpose: Histone H2AK119ub antibody (pAb)

Purification: Affinity Purified

Immunogen: This antibody was raised against a branched peptide corresponding to the ubiquitin conjugation site at Lys119 of human Histone H2A.

Isotype: IgG

Application Details

Application Notes: DB: 1:1000 WB*: 1:1000 - 1:2000 dilution *Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. For optimal results, we recommend the addition of 0.05 % Tween-20 to all blocking solutions to reduce background. Individual optimization may be required.

Restrictions: For Research Use only

Handling

Buffer: 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol, and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for Histone H2A

Target: Histone H2A

Background: In humans, attachment of ubiquitin to Histone protein 2A (H2A) on lysine residue 119 (H2AK119Ub) is one of the most prevalent histone marks, and is estimated to occur on 5-15 % of histones genome-wide4. In general, H2AK119Ub is characteristic of repressed developmental genes, and is also tightly linked to levels of other histone marks such as lysine methylation and acetylation. H2AK119Ub is attached to histones by the Really Interesting New Gene (RING) ubiquitin E3-ligase Polycomb Repressive Complex 1 (PRC1), and removed by the Polycomb Repressive-Deubiquitinase (PR-DUB) complex, which plays a vital role in regulating H2AK119Ub histone marks, and mutations in human PR-DUB genes lead to a spectrum of malignancies.

Molecular Weight: 23 kDa

NCBI Accession: NP_003508