Histone H2A antibody (AA 122-129)

Catalog No. ABIN7938167

This Rabbit Polyclonal antibody specifically detects Histone H2A in WB and DB. It exhibits reactivity toward Human and Mouse.

Quick Overview for Histone H2A antibody (AA 122-129) (ABIN7938167)

Target: Histone H2A

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H2A antibody is un-conjugated

Application: Western Blotting (WB), Dot Blot (DB)

Product Details anti-Histone H2A Antibody

Binding Specificity: AA 122-129

Purpose: Histone H2A.J antibody (pAb)

Purification: Affinity Purified

Immunogen: This antibody was raised against a peptide comprising amino acids 122-129 of human Histone H2A.J.

Isotype: IgG

Application Details

Application Notes: WB*: 1:500 - 1:2500 dilution DB: 1:1000 dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for Histone H2A

Target: Histone H2A

Alternative Name: Histone H2A.

Background: Histone H2A is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, and is responsible for establishing higher-order chromatin structure. Histone H2A.J is a histone H2A variant, a protein similar to canonical H2A but with different molecular identity and unique functions. Histone H2A.J, which is found only in mammals, is shown to be associated with senescence, accumulating in senescent fibroblasts and promoting expression of inflammatory genes. Therefore, H2A.J may contribute to chronic inflammation and age-related diseases.

NCBI Accession: NP_808760