Histone H2A antibody (C-Term)

Catalog No. ABIN7938168

The Mouse Monoclonal anti-Histone H2A antibody (Clone 10E9-D1) (ABIN7938168) specifically detects Histone H2A in WB and ChIP. The antibody is reactive with Drosophila melanogaster samples.

Quick Overview for Histone H2A antibody (C-Term) (ABIN7938168)

Target: Histone H2A

Reactivity: Drosophila melanogaster

Host: Mouse

Clonality: Monoclonal

Conjugate: This Histone H2A antibody is un-conjugated

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)

Clone: 10E9-D1

Product Details anti-Histone H2A Antibody

Binding Specificity: C-Term

Purpose: Histone H2Av antibody (mAb) (Clone 10E9.D1), sample

Purification: Protein A Chromatography

Immunogen: This antibody was raised against a peptide in the C-terminus of the Drosophila melanogaster Histone variant H2Av.

Isotype: IgG2a

Application Details

Application Notes: ChIP: 5 μg per ChIP WB: 0.5 - 2 μg/mL dilution

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for Histone H2A

Target: Histone H2A

Background: The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H2A.Z (H2AZ, H2AFZ) is a histone H2A variant, a protein similar to canonical H2A but with different molecular identity and unique functions. H2A.Z is highly conserved during evolution. It plays an important role in basic cellular mechanisms such as gene activation, chromosome segregation, heterochromatic silencing and progression through the cell cycle.In Drosophila, the H2A variant corresponding to H2AZ is H2Av. H2Av is an essential protein in Drosophila and has been implicated in both activation and repression of transcription. H2Av is localized to centromeric heterochromatin in Drosophila and flies lacking H2Av have reduced levels of heterochromatin components at the centromeres. However, H2Av nucleosome distribution throughout the rest of the Drosophila genome correlates with genes that have an open and uniform chromatin architecture at promoter regions.

Molecular Weight: 15 kDa

NCBI Accession: NP_524519