Recombinant Histone H2A antibody (H2A.XS139ph)

Catalog No. ABIN7938169

This Rabbit Monoclonal antibody specifically detects Histone H2A in WB and ICC. It exhibits reactivity toward Human.

Quick Overview for Recombinant Histone H2A antibody (H2A.XS139ph) (ABIN7938169)

Target: Histone H2A

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Histone H2A antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Clone: RM224

Product Details anti-Histone H2A Antibody

Binding Specificity: H2A.XS139ph

Purpose: Histone H2A.XS139ph antibody (rAb) (H2AX) (Clone RM224)

Purification: Protein A Chromatography

Immunogen: A phospho-peptide corresponding to Phospho-Histone H2AX (Ser139).

Isotype: IgG

Application Details

Application Notes: WB: 0.5 - 2 μg/mL ICC: 0.5 - 2 μg/mL

Restrictions: For Research Use only

Handling

Buffer: PBS with 50 % glycerol, 1 % BSA, and 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for Histone H2A

Target: Histone H2A

Alternative Name: Histone H2A.

Background: Histone H2AX phospho Ser139 (H2AX, H2A histone family member X) replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries that require DNA as a template. Histones thereby play a central role in transcriptional regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called the histone code, and nucleosome remodeling. Histone H2AX is required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation, and for efficient repair of DNA double-strand breaks (DSBs), specifically when modified by C-terminal phosphorylation. RM224 reacts to Histone H2A.X only when phosphorylated at serine 139. No cross reactivity with other phosphorylated histones.

NCBI Accession: NP_002096