Histone H2A antibody (C-Term)

Catalog No. ABIN7938170

The Rabbit Polyclonal anti-Histone H2A antibody is suitable to detect Histone H2A in samples from Human. It has been validated for WB, IF, DB, ChIP and ChIP-seq.

Quick Overview for Histone H2A antibody (C-Term) (ABIN7938170)

Target: Histone H2A

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H2A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Dot Blot (DB), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq)

Product Details anti-Histone H2A Antibody

Binding Specificity: C-Term

Purpose: Histone H2A.Z antibody (pAb) (H2AZ)

Purification: Unpurified

Immunogen: This Histone H2A.Z antibody was raised against a peptide derived from the C-terminus of human histone H2A.Z.

Application Details

Application Notes: ChIP: 2 - 10 µL per ChIP ChIP-Seq: 5 µL each WB*: 1:1,000 - 1:5,000 dilution This histone variant can be perturbed by excessive washing steps prior to fixation. Cells should be washed into serum-free culture medium (NOT PBS!) prior to fixation. *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Restrictions: For Research Use only

Handling

Buffer: Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for Histone H2A

Target: Histone H2A

Alternative Name: Histone H2A.

Background: The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. Histone H2A.Z (H2AZ, H2AFZ) is a histone H2A variant, a protein similar to canonical H2A but with different molecular identity and unique functions. H2A.Z is highly conserved during evolution. It plays an important role in basic cellular mechanisms such as gene activation, chromosome segregation, heterochromatic silencing and progression through the cell cycle. H2A.Z is acetylated at multiple lysine residues in its amino terminus, which may enable H2A.Z to function as an insulator of chromatin domains.

Molecular Weight: 14 kDa

NCBI Accession: NP_002097