KDM5B antibody

Catalog No. ABIN7938293

The Rabbit Polyclonal anti-KDM5B antibody (ABIN7938293) specifically detects KDM5B in ChIP. The antibody is reactive with Human samples.

Quick Overview for KDM5B antibody (ABIN7938293)

Target: KDM5B (Lysine (K)-Specific Demethylase 5B (KDM5B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KDM5B antibody is un-conjugated

Application: Chromatin Immunoprecipitation (ChIP)

Product Details anti-KDM5B Antibody

Purpose: JARID1B / KDM5B antibody (pAb)

Purification: Protein A Chromatography

Immunogen: This antibody was raised against a human peptide belonging to demethyase JARID1B.

Isotype: IgG

Application Details

Application Notes: ChIP-Seq: 10 µL each

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS, 30 % glycerol, 0.035 % Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for KDM5B

Target: KDM5B (Lysine (K)-Specific Demethylase 5B (KDM5B))

Alternative Name: JARID1B

Background:

Background_Plain: Lysine (K)-specific demethylase 5B (KDM5B), also known as Jumonji, AT rich interactive domain 1B (JARID1B), is a histone demethylase that specifically demethylates lysine 4 of histone H3 (H3K4), thereby playing a central role in defining the histone code. KDM5B demethylates trimethylated, dimethylated and monomethylated H3K4. It does not demethylate histone H3K9 or H3K27. KDM5B functions as a transcriptional corepressor for FOXG1B and PAX9. KDM5B induces the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5. In contrast, it may act as a tumor suppressor for melanoma.

Aliases: Lysine Demethylase 5B, lysine (K)-specific demethylase 5B

Molecular Weight: 180 kDa

NCBI Accession: NP_006609

Pathways: Warburg Effect