METTL14 antibody

Catalog No. ABIN7938341

This Rabbit Polyclonal antibody specifically detects METTL14 in WB and IP. It exhibits reactivity toward Human.

Quick Overview for METTL14 antibody (ABIN7938341)

Target: METTL14 (Methyltransferase Like 14 (METTL14))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This METTL14 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-METTL14 Antibody

Purpose: METTL14 antibody (pAb)

Purification: Affinity Purified

Immunogen: This antibody was raised against a human peptide containing methyltransferase 14.

Isotype: IgG

Application Details

Application Notes: WB: 1:500 - 1:2000 dilution IP: 4 µL per IP

Restrictions: For Research Use only

Handling

Buffer: Purified in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for METTL14

Target: METTL14 (Methyltransferase Like 14 (METTL14))

Alternative Name: METTL14

Background:

Background_Plain: N6-methylated adenine (m6A) is prevalently present in nearly all RNA types and can be found in all organisms from bacteria to humans. It preferentially appears around stop codons and within long internal exons in mammalian messager RNAs. m6A plays an important role in the efficiency of mRNA splicing, processing, translation efficiency, editing and mRNA stability. m6A also takes place in other RNA molecules, such as primary miRNA (pri-miRNAs). METTL14 (methyltransferase-like 14)forms a stable N6-methyltransferase heterodimer complex with METTL3, which catalyzes the generation of m6A modification on mammalian nuclear RNAs. METTL3 is the catalytically active subunit, while METTL14 plays a structural role critical for substrate recognition.

Aliases: Methyltransferase Like 14, EC 2.1.1.62, N6-methyltransferase

Molecular Weight: 55kDa

NCBI Accession: NP_066012