MINA antibody (C-Term)

Catalog No. ABIN7938352

The Rabbit Polyclonal anti-MINA antibody (ABIN7938352) specifically detects MINA in WB. The antibody is reactive with Human samples.

Quick Overview for MINA antibody (C-Term) (ABIN7938352)

Target: MINA (MYC Induced Nuclear Antigen (MINA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MINA antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MINA Antibody

Binding Specificity: C-Term

Purpose: MINA antibody (pAb)

Purification: Affinity Purified

Immunogen: This antibody was raised against a peptide within the C-terminal region of human MINA.

Isotype: IgG

Application Details

Application Notes: WB*: 1:500 - 1:2,000 dilution The addition of 0.1 % Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for MINA

Target: MINA (MYC Induced Nuclear Antigen (MINA))

Alternative Name: MINA

Background:

Background_Plain: MINA (MYC Induced Nuclear Antigen) is an oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. Is involved in the demethylation of trimethylated Lys-9 on histone H3 (H3K9me3), leading to an increase in ribosomal RNA expression. Also catalyzes the hydroxylation of 60S ribosomal protein L27a on His-39. May play an important role in cell growth and survival. May be involved in ribosome biogenesis, most likely during the assembly process of pre-ribosomal particles.

Aliases: ROX, MDIG, NO52, MINA53

Molecular Weight: 55 kDa

NCBI Accession: NP_694822