NSUN2 antibody

Catalog No. ABIN7938379

This Rabbit Polyclonal antibody specifically detects NSUN2 in WB. It exhibits reactivity toward Human.

Quick Overview for NSUN2 antibody (ABIN7938379)

Target: NSUN2 (NOP2/Sun Domain Family, Member 2 (NSUN2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NSUN2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NSUN2 Antibody

Purpose: NSUN2 antibody (pAb)

Purification: Protein A Chromatography

Immunogen: Peptide within the human NSUN2 (RNA cytosine C(5)-methyltransferase NSUN2) isoform 1.

Isotype: IgG

Application Details

Application Notes: WB: 0.5 - 2.0 μg/mL dilution

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for NSUN2

Target: NSUN2 (NOP2/Sun Domain Family, Member 2 (NSUN2))

Alternative Name: NSUN2

Background:

Background_Plain: NSUN2 (NOP2/Sun Domain Family, Member 2), also called TRM4 (TRNA Methyltransferase 4 Homolog), is a member of NSUN family. It's a RNA methyltransferase that methylates tRNAs, and possibly RNA polymerase III transcripts. NSUN2 also methylates cytosine to 5-methylcytosine (m5C) at positions 34 and 48 of intron containing. tRNA(Leu)(CAA) precursors, and at positions 48, 49 and 50 of tRNA(Gly)(GCC) precursors. It may act downstream of Myc to regulate epidermal cell growth and proliferation.NSUN2 is required for proper spindle assembly and chromosome segregation, independently of its methyltransferase activity.

Aliases: NOP2/Sun RNA Methyltransferase Family Member 2, Myc-Induced SUN-Domain-Containing Protein, MISU, SAKI, MRT5

Molecular Weight: 100 kDa

NCBI Accession: NP_060225